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Title: | On the Fine Isotopic Distribution and Limits to Resolution in Mass Spectrometry | Authors: | Dittwald, Piotr VALKENBORG, Dirk CLAESEN, Jurgen Rockwood, Alan Gambin, Anna |
Issue Date: | 2015 | Source: | Journal of the American Society for Mass Spectrometry, 26 (10), p. 1732-1745 | Abstract: | Mass spectrometry enables the study of increasingly larger biomolecules with increasingly higher resolution, which is able to distinguish between fine isotopic variants having the same additional nucleon count, but slightly different masses. Therefore, the analysis of the fine isotopic distribution becomes an interesting research topic with important practical applications. In this paper, we propose the comprehensive methodology for studying the basic characteristics of the fine isotopic distribution. Our approach uses a broad spectrum of methods ranging from generating functions—that allow us to estimate the variance and the information theory entropy of the distribution—to the theory of thermal energy fluctuations. Having characterized the variance, spread, shape, and size of the fine isotopic distribution, we are able to indicate limitations to high resolution mass spectrometry. Moreover, the analysis of “thermorelativistic” effects (i.e., mass uncertainty attributable to relativistic effects coupled with the statistical mechanical uncertainty of the energy of an isolated ion), in turn, gives us an estimate of impassable limits of isotopic resolution (understood as the ability to distinguish fine structure peaks), which can be moved further only by cooling the ions. The presented approach highlights the potential of theoretical analysis of the fine isotopic distribution, which allows modeling the data more accurately, aiming to support the successful experimental measurements. | Notes: | Correspondence to: Alan Rockwood; e-mail: alan.rockwood@aruplab.com, Anna Gambin; e-mail: aniag@mimuw.edu.pl | Keywords: | fine isotopic distribution; thermorelativistic effect; limits to resolution; mathematical modeling | Document URI: | http://hdl.handle.net/1942/19697 | ISSN: | 1044-0305 | e-ISSN: | 1879-1123 | DOI: | 10.1007/s13361-015-1180-4 | ISI #: | 000361073200012 | Rights: | © The Author(s), 2015. This article is published with open access at Springerlink.com. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2016 |
Appears in Collections: | Research publications |
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On the fine isotopic distribution and limits to resolution in mass spectrometry - JASMS 2015.pdf | 1.34 MB | Adobe PDF | View/Open |
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