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Title: | Genetic variation in TLR10 is not associated with chronic Q fever, despite the inhibitory effect of TLR10 on Coxiella burnetii-induced cytokines in vitro | Authors: | Ammerdorffer, Anne STAPPERS, Mark Oosting, Marije Schoffelen, Teske Hagenaars, Julia C. J. P. Bleeker-Rovers, Chantal P. Wegdam-Blans, Marjolijn C. Wever, Peter C. Roest, Hendrik-Jan van de Vosse, Esther Netea, Mihai G. Sprong, Tom Joosten, Leo A. B. |
Issue Date: | 2016 | Publisher: | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Source: | CYTOKINE, 77, p. 196-202 | Abstract: | Coxiella burnetii, the causative agent of Q fever, is recognized by TLR2. TLR10 can act as an inhibitory receptor on TLR2-derived immune responses. Therefore, we investigated the role of TLR10 on C. burnetii-induced cytokine production and assessed whether genetic polymorphisms in TLR10 influences the development of chronic Q fever. HEK293 cells, transfected with TLR2, TLR10 or TLR2/TLR10, and human peripheral blood mononuclear cells (PBMCs) in the presence of anti-TLR10, were stimulated with C burnetii. In both assays, the absence of TLR10 resulted in increased cytokine responses after C burnetii stimulation. In addition, the effect of single nucleotide polymorphisms (SNPs) in TLR10 was examined in healthy volunteers whose PBMCs were stimulated with C burnetii Nine Mile or the Dutch outbreak isolate C burnetii 3262. Individuals bearing SNPs in TLR10 displayed increased cytokine production upon C burnetii 3262 stimulation. Furthermore, 139 chronic Q fever patients and 220 controls were genotyped for TLR10 N241H, 1775V and I369L. None of these polymorphisms were associated with increased susceptibility to chronic Q fever. In conclusion, TLR10 has an inhibitory effect on in vitro cytokine production by C. burnetii, but the presence of TLR10 polymorphisms does not lead to an increased risk of developing chronic Q fever. (C) 2015 Elsevier Ltd. All rights reserved. | Notes: | [Ammerdorffer, Anne; Stappers, Mark H. T.; Oosting, Marije; Schoffelen, Teske; Bleeker-Rovers, Chantal P.; Netea, Mihai G.; Sprong, Tom; Joosten, Leo A. B.] Radboud Univ Nijmegen, Med Ctr, Dept Internal Med, NL-6525 GA Nijmegen, Netherlands. [Ammerdorffer, Anne; Roest, Hendrik-Jan] Cent Vet Inst, Dept Bacteriol & TSEs, NL-8219 PH Lelystad, Netherlands. [Stappers, Mark H. T.] Hasselt Univ, B-3500 Hasselt, Belgium. [Stappers, Mark H. T.; Sprong, Tom] Canisius Wilhelmina Ziekenhuis, Dept Med Microbiol & Infect Dis, NL-6532 SZ Nijmegen, Netherlands. [Hagenaars, Julia C. J. P.] Jeroen Bosch Hosp, Dept Surg, NL-5223 GW Shertogenbosch, Netherlands. [Wegdam-Blans, Marjolijn C.] Lab Pathol & Med Microbiol PAMM, Dept Med Microbiol, NL-5504 DL Veldhoven, Netherlands. [Wever, Peter C.] Jeroen Bosch Hosp, Dept Med Microbiol & Infect Control, NL-5223 GW Shertogenbosch, Netherlands. [van de Vosse, Esther] Leiden Univ, Med Ctr, Dept Infect Dis, NL-2333 ZA Leiden, Netherlands. [Sprong, Tom] Canisius Wilhelmina Ziekenhuis, Dept Internal Med, NL-6532 SZ Nijmegen, Netherlands. | Keywords: | Q fever; Coxiella burnetii; Toll-like receptor 10; Single nucleotide polymorphism; Innate immunity;Q fever; coxiella burnetii; toll-like receptor 10; single nucleotide polymorphism; innate immunity | Document URI: | http://hdl.handle.net/1942/20606 | ISSN: | 1043-4666 | e-ISSN: | 1096-0023 | DOI: | 10.1016/j.cyto.2015.09.005 | ISI #: | 000366540500025 | Rights: | © 2015 Elsevier Ltd. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
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