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Title: | Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice | Authors: | Holvoet, Bryan Quattrocelli, Mattia Belderbos, Sarah Pollaris, Lore WOLFS, Esther Gheysens, Olivier Gijsbers, Rik Vanoirbeek, Jeroen Verfaillie, Catherine M. Sampaolesi, Maurilio Deroose, Christophe M. |
Issue Date: | 2015 | Publisher: | CELL PRESS | Source: | STEM CELL REPORTS, 5 (6), p. 1183-1195 | Abstract: | Muscular dystrophies are a heterogeneous group of myopathies, characterized by muscle weakness and degeneration, without curative treatment. Mesoangioblasts (MABs) have been proposed as a potential regenerative therapy. To improve our understanding of the in vivo behavior of MABs and the effect of different immunosuppressive therapies, like cyclosporine A or co-stimulation-adhesion blockade therapy, on cell survival noninvasive cell monitoring is required. Therefore, cells were transduced with a lentiviral vector encoding firefly luciferase (Fluc) and the human sodium iodide transporter (hNIS) to allow cell monitoring via bioluminescence imaging (BLI) and small-animal positron emission tomography (PET). Non-H2 matched mMABs were injected in the femoral artery of dystrophic mice and were clearly visible via small-animal PET and BLI. Based on noninvasive imaging data, we were able to show that co-stim was clearly superior to CsA in reducing cell rejection and this was mediated via a reduction in cytotoxic Tcells and upregulation of regulatory Tcells. | Notes: | [Holvoet, Bryan; Belderbos, Sarah; Gheysens, Olivier; Deroose, Christophe M.] Katholieke Univ Leuven, Ctr Environm & Hlth, Dept Imaging & Pathol Nucl Med & Mol Imaging, B-3000 Leuven, Belgium. [Quattrocelli, Mattia; Sampaolesi, Maurilio] Katholieke Univ Leuven, Ctr Environm & Hlth, Translat Cardiomyol Lab, Dept Dev & Regenerat, B-3000 Leuven, Belgium. [Pollaris, Lore; Vanoirbeek, Jeroen] Katholieke Univ Leuven, Ctr Environm & Hlth, Dept Publ Hlth & Primary Care, B-3000 Leuven, Belgium. [Wolfs, Esther] Univ Hasselt, Histol Lab, Biomed Res Inst, Dept Morphol, B-3590 Diepenbeek, Belgium. [Gijsbers, Rik] Katholieke Univ Leuven, Stem Cell Inst Leuven, Lab Mol Virol & Gene Therapy, Dept Pharmaceut & Pharmacol Sci,Leuven Viral Vect, B-3000 Leuven, Belgium. [Vanoirbeek, Jeroen] Katholieke Univ Leuven, Stem Cell Inst Leuven, Dept Dev & Regenerat, B-3000 Leuven, Belgium. | Document URI: | http://hdl.handle.net/1942/20692 | ISSN: | 2213-6711 | e-ISSN: | 2213-6711 | DOI: | 10.1016/j.stemcr.2015.10.018 | ISI #: | 000366142900022 | Rights: | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
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holvoet 1.pdf | Published version | 3.17 MB | Adobe PDF | View/Open |
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