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http://hdl.handle.net/1942/20908
Title: | Serological diagnosis of bovine neosporosis: a Bayesian evaluation of two Antibody ELISA tests for in vivo diagnosis in purchased an abortion cattle. | Authors: | Roelandt, S. Van der Stede, Y. Czaplicki, G. Van Loo, H. Van Driessche, E. Dewulf, J. HOOYBERGHS, Jef FAES, Christel |
Issue Date: | 2015 | Source: | Veterinary record 176(23) | Abstract: | Currently, there are no perfect reference tests for the in vivo detection of Neospora caninum infection. Two commercial N caninum ELISA tests are currently used in Belgium for bovine sera (TEST A and TEST B). The goal of this study is to evaluate these tests used at their current cut-offs, with a no gold standard approach, for the test purpose of (1) demonstration of freedom of infection at purchase and (2) diagnosis in aborting cattle. Sera of two study populations, Abortion population (n=196) and Purchase population (n=514), were selected and tested with both ELISA's. Test results were entered in a Bayesian model with informative priors on population prevalences only (Scenario 1). As sensitivity analysis, two more models were used: one with informative priors on test diagnostic accuracy (Scenario 2) and one with all priors uninformative (Scenario 3). The accuracy parameters were estimated from the first model: diagnostic sensitivity (Test A: 93.54 per cent–Test B: 86.99 per cent) and specificity (Test A: 90.22 per cent–Test B: 90.15 per cent) were high and comparable (Bayesian P values >0.05). Based on predictive values in the two study populations, both tests were fit for purpose, despite an expected false negative fraction of ±0.5 per cent in the Purchase population and ±5 per cent in the Abortion population. In addition, a false positive fraction of ±3 per cent in the overall Purchase population and ±4 per cent in the overall Abortion population was found. | Document URI: | http://hdl.handle.net/1942/20908 | ISSN: | 0042-4900 | e-ISSN: | 2042-7670 | DOI: | 10.1136/vr.102872 | ISI #: | 000356167400018 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2016 |
Appears in Collections: | Research publications |
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Veterinary Record-2015-Roelandt-vr.102872.pdf Restricted Access | Published version | 552.86 kB | Adobe PDF | View/Open Request a copy |
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