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http://hdl.handle.net/1942/21453
Title: | Heat-transfer based characterization of DNA on synthetic sapphire chips | Authors: | MURIB, Mohammed Sharif YEAP, Weng Siang Eurlings, Y. VAN GRINSVEN, Bart BOYEN, Hans-Gerd CONINGS, Bert MICHIELS, Luc AMELOOT, Marcel CARLEER, Robert Warmer, J. Kaul, P. HAENEN, Ken Schoening, M.J. DE CEUNINCK, Ward WAGNER, Patrick |
Issue Date: | 2016 | Source: | SENSORS AND ACTUATORS B-CHEMICAL, 230, p. 260-271 | Abstract: | In this study, we show that synthetic sapphire (Al2O3), an established implant material, can also serve as a platform material for biosensors comparable to nanocrystalline diamond. Sapphire chips, beads, and powder were first modified with (3-aminopropyl) triethoxysilane (APTES), followed by succinic anhydride (SA), and finally single-stranded probe DNA was EDC coupled to the functionalized layer. The presence of the APTES-SA layer on sapphire powders was confirmed by thermogravimetric analyis and Fourier-transform infrared spectroscopy. Using planar sapphire chips as substrates and X-ray photoelectron spectroscopy (XPS) as surface-sensitive tool, the sequence of individual layers was analyzed with respect to their chemical state, enabling the quantification of areal densities of the involved molecular units. Fluorescence microscopy was used to demonstrate the hybridization of fluorescently tagged target DNA to the probe DNA, including denaturation- and re-hybridization experiments. Due to its high thermal conductivity, synthetic sapphire is especially suitable as a chip material for the heat-transfer method, which was employed to distinguish complementary- and non-complementary DNA duplexes containing single-nucleotide polymorphisms. These results indicate that it is possible to detect mutations electronically with a chemically resilient and electrically insulating chip material. | Notes: | Murib, MS (reprint author), Univ Ghent, B-9000 Ghent, Belgium. mohammed.murib@aum.edu.kw | Keywords: | synthetic sapphire; nanocrystalline diamond; heat-transfer method; fourier-transform infrared spectroscopy; thermogravimetric analysis; X-ray photoelectron spectroscopy; confocal fluorescence microscopy; single-nucleotide polymorphisms in DNA | Document URI: | http://hdl.handle.net/1942/21453 | e-ISSN: | 0925-4005 | DOI: | 10.1016/j.snb.2016.02.027 | ISI #: | 000374329300033 | Rights: | © 2016 Elsevier B.V. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
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1-s2.0-S0925400516301770-main.pdf Restricted Access | Published version | 2.34 MB | Adobe PDF | View/Open Request a copy |
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