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Title: | Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy | Authors: | BOVE, Hannelore Steuwe, Christian Fron, Eduard SLENDERS, Eli D'HAEN, Jan Fujita, Yasuhiko Uji-i, Hiroshi VAN DE VEN, Martin Roeffaers, Maarten AMELOOT, Marcel |
Issue Date: | 2016 | Publisher: | AMER CHEMICAL SOC | Source: | NANO LETTERS, 16 (5), p. 3173-3178 | Abstract: | Although adverse health effects of carbon black (CB) exposure are generally accepted, a direct, label-free approach for detecting CB particles in fluids and at the cellular level is still lacking. Here, we report nonincandescence related white-light (WL) generation by dry and suspended carbon black particles under illumination with femtosecond (fs) pulsed near-infrared light as a powerful tool for the detection of these carbonaceous materials. This observation is done for four different CB species with diameters ranging from 13 to 500 nm, suggesting this WL emission under fs near-infrared illumination is a general property of CB particles. As the emitted radiation spreads over the whole visible spectrum, detection is straightforward and flexible. The unique property of the described WL emission allows optical detection and unequivocal localization of CB particles in fluids and in cellular environments while simultaneously colocalizing different cellular components using various specific fluorophores as shown here using human lung fibroblasts. The experiments are performed on a typical multiphoton laser-scanning microscopy platform, widely available in research laboratories. | Notes: | [Bove, Hannelore; Slenders, Eli; vandeVen, Martin; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Bove, Hannelore; Steuwe, Christian; Roeffaers, Maarten] Katholieke Univ Leuven, Ctr Surface Chem & Catalysis, Celestijnenlaan 200F, B-3001 Leuven, Belgium. [Fron, Eduard; Fujita, Yasuhiko; Uji-i, Hiroshi] Katholieke Univ Leuven, Dept Chem, Celestijnenlaan 200F, B-3001 Leuven, Belgium. [D'Haen, Jan] Hasselt Univ, Inst Mat Res, Wetenschapspk 1, B-3950 Diepenbeek, Belgium. [Uji-i, Hiroshi] Hokkaido Univ, Res Inst Elect Sci, Kita Ward, N20W10, Sapporo, Hokkaido 0010020, Japan. | Keywords: | carbon black particles; label-free detection in aqueous environments; white light emission; femtosecond pulsed laser illumination; human lung fibroblasts;Carbon black particles; label-free detection in aqueous environments; white light emission; femtosecond pulsed laser illumination; human lung fibroblasts | Document URI: | http://hdl.handle.net/1942/21558 | ISSN: | 1530-6984 | e-ISSN: | 1530-6992 | DOI: | 10.1021/acs.nanolett.6b00502 | ISI #: | 000375889700042 | Rights: | This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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acs%2Enanolett%2E6b00502.pdf | Published version | 4.7 MB | Adobe PDF | View/Open |
Biocompatible Label-free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy.pdf | Peer-reviewed author version | 1.18 MB | Adobe PDF | View/Open |
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