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Title: | Bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections: direct molecular versus culture-based detection | Authors: | STAPPERS, Mark Hagen, Ferry Reimnitz, Peter Mouton, Johan W. Meis, Jacques F. GYSSENS, Inge |
Issue Date: | 2016 | Publisher: | ELSEVIER SCIENCE INC | Source: | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 85 (2), p. 263-265 | Abstract: | Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. Real-time PCR resulted in an increased detection rate of 12% for B. fragilis and could improve the detection of B. fragilis in clinical samples. (C) 2016 Elsevier Inc. All rights reserved. | Notes: | [Stappers, Mark H. T.; Gyssens, Inge C.] Radboud Univ Nijmegen, Dept Internal Med, Med Ctr, Geert Grootepl Zuid 10, NL-6525 GA Nijmegen, Netherlands. [Stappers, Mark H. T.; Hagen, Ferry; Meis, Jacques F.; Gyssens, Inge C.] Canisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, Weg Door Jonkerbos 100, NL-6532 SZ Nijmegen, Netherlands. [Stappers, Mark H. T.; Gyssens, Inge C.] Hasselt Univ, Martelarenlaan 42, BE-3500 Hasselt, Belgium. [Reimnitz, Peter] Bayer Healthcare Pharmaceut, Friedrich Ebert Str 475, D-42117 Wuppertal, Germany. [Mouton, Johan W.; Meis, Jacques F.] Radboud Univ Nijmegen, Med Ctr, Dept Med Microbiol, Geert Grootepl Zuid 10, NL-6525 GA Nijmegen, Netherlands. [Mouton, Johan W.; Meis, Jacques F.] Erasmus MC, Dept Med Microbiol & Infect Dis, S Gravendijkwal 230, NL-3015 CE Rotterdam, Netherlands. | Keywords: | acute bacterial skin and skin structure infections; bacteroides fragilis; real-time polymerase chain reaction; culture; microbiology; diagnosis;Acute bacterial skin and skin structure infections; Bacteroides fragilis; Real-time polymerase chain reaction; Culture; Microbiology; Diagnosis | Document URI: | http://hdl.handle.net/1942/21593 | ISSN: | 0732-8893 | e-ISSN: | 1879-0070 | DOI: | 10.1016/j.diagmicrobio.2015.12.017 | ISI #: | 000376822600030 | Rights: | © 2016 Elsevier Inc. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
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