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Title: The in planta proteome of wild type strains of the fire blight pathogen, Erwinia amylovora
Authors: HOLTAPPELS, Michelle 
Vrancken, Kristof
NOBEN, Jean-Paul 
Schoofs, H.
Deckers, T.
VALCKE, Roland 
Issue Date: 2016
Source: JOURNAL OF PROTEOMICS, 139, p. 1-12
Abstract: Erwinia amylovora is a Gram-negative plant pathogen that causes fire blight. This disease affects most members of the Rosaceae family including apple and pear. Here, an infection model is introduced to study proteomic changes in a highly virulent E. amylovora strain upon interaction with its host as compared to a lower virulent strain. For this purpose separate shoots of apple rootstocks were wound-infected and when infection became systemic, bacterial cells were isolated and processed for analysis in a proteomics platform combining 2-D fluorescence difference gel electrophoresis and mass spectrometry. Comparing the proteome of the isolates, significant abundance changes were observed in proteins involved in sorbitol metabolism, amylovoran production as well as in protection against plant defense mechanisms. Furthermore several proteins associated with virulence were more abundant in the higher virulent strain. Changes at the proteome level showed good accordance at the transcript level, as was verified by RT-qPCR. In conclusion, this infection model may be a valuable tool to unravel the complexity of plant-pathogen interactions and to gain insight in the molecular mechanisms associated with virulence of E. amylovora, paving the way for the development of plant-protective interventions against this detrimental disease. Significance: During this research a first time investigation was performed on the proteome of E. amylovora, grown inside a susceptible host plant. This bacterium is the causal agent of fire blight, which can affect most members of the Rosaceae family including apple and pear. To do so, an artificial infection model on shoots of apple rootstocks was optimized and employed. When infection was systemic, bacterial cells were extracted from the plant tissue followed by extraction of the proteins from the bacteria. Further processing of the proteins was done by using a 2-D fluorescence difference gel electrophoresis analysis followed by mass spectrometry. By the use of two strains differing in their virulent ability, we were able to draw conclusions concerning virulence and behavior of different strains inside the host. This research provides a model to investigate plant-pathogen interactions and more importantly, we identified possible new targets for the development of novel control methods against this devastating disease. (C) 2016 Elsevier B.V. All rights reserved.
Notes: [Holtappels, M.; Valcke, R.] Hasselt Univ, Fac Sci, Mol & Phys Plant Physiol, Hasselt, Belgium. [Vrancken, K.] PCFruit Res Stn, Dept Zool, Hasselt, Belgium. [Noben, J. P.] Hasselt Univ, Biomed Res Inst, Hasselt, Belgium. [Noben, J. P.] Hasselt Univ, Transnat Univ Limburg, Sch Life Sci, Hasselt, Belgium. [Remans, T.] Hasselt Univ, Ctr Environm Sci, Hasselt, Belgium. [Schoofs, H.; Deckers, T.] PCFruit Res Stn, Dept Pomol, Hasselt, Belgium.
Keywords: Erwinia amylovora; 2D DIGE; In planta; Plant-pathogen interactions; Amylovoran; Sorbitol;erwinia amylovora; 2D DIGE; in planta; plant-pathogen interactions; amylovoran; sorbitol
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ISSN: 1874-3919
e-ISSN: 1876-7737
DOI: 10.1016/j.jprot.2016.02.018
ISI #: 000375164600001
Rights: © 2016 Elsevier B.V. All rights reserved.
Category: A1
Type: Journal Contribution
Validations: ecoom 2017
Appears in Collections:Research publications

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