Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/21835
Title: | Impedimetric immunosensor for the detection of histamine based on reduced graphene oxide | Authors: | DELLE, Lotta HUCK, Christina Baecker, Matthias Mueller, Frank Grandthyll, Samuel Jacobs, Karin Lilischkis, Rainer Vu, Xuan T. Schoening, Michael J. WAGNER, Patrick THOELEN, Ronald Weil, Maryam Ingebrandt, Sven |
Issue Date: | 2015 | Publisher: | WILEY-V C H VERLAG GMBH | Source: | PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 212 (6), p. 1327-1334 | Abstract: | In this work, we present a reduced graphene oxide-based impedimetric histamine biosensor. Histamine as a bioamine mediates immune-modulatory effects in acute inflammatory and allergic reactions. In a proof-of-principle approach, the low-cost sensor platform determines low concentrations of histamine in buffer solution with an impedimetric readout technique. As a sensor platform we used ultra-thin graphene oxide lines fabricated by a simple soft-lithographic approach. The lines of about 20m width were characterised by atomic force microscopy and scanning electron microscopy. Reduction of graphene oxide to a conductive transducer material was performed by a rapid thermal protocol in inert atmosphere. The reduction process was monitored by impedance spectroscopy and X-ray photoelectron spectroscopy. For our biosensor experiments we adopted a commonly-used protocol for surface plasmon resonance (SPR) detection of histamine. With our reduced graphene oxide platform we detected antigen-antibody binding events of histamine with concentrations of 0.1-1M via impedance changes in a low frequency range. The results from the rGO sensors were comparable to the SPR detection. | Notes: | [Delle, Lotta E.; Lilischkis, Rainer; Vu, Xuan T.; Weil, Maryam; Ingebrandt, Sven] Univ Appl Sci Kaiserslautern, Dept Informat & Microsyst Technol, D-66482 Zweibrucken, Germany. [Huck, Christina; Baecker, Matthias; Schoening, Michael J.] FH Aachen, INB, D-52428 Julich, Germany. [Mueller, Frank; Grandthyll, Samuel; Jacobs, Karin] Univ Saarland, Expt Phys, D-66123 Saarbrucken, Germany. [Vu, Xuan T.] Rhein Westfal TH Aachen, Dept Phys IA, D-52074 Aachen, Germany. [Wagner, Patrick] Katholieke Univ Leuven, Dept Soft Matter & Biophys, B-3001 Leuven, Belgium. [Wagner, Patrick; Thoelen, Ronald] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium. | Keywords: | histamine; immunosensors; impedance spectroscopy; reduced graphene oxide;histamine; immunosensors; impedance spectroscopy; reduced graphene oxide | Document URI: | http://hdl.handle.net/1942/21835 | ISSN: | 1862-6300 | e-ISSN: | 1862-6319 | DOI: | 10.1002/pssa.201431863 | ISI #: | 000356706500022 | Rights: | © 2015 WILEY VCH Verlag GmbH & Co. KGaA, Weinheim | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2016 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Delle_et_al-2015-physica_status_solidi_(a).pdf Restricted Access | Published version | 745.01 kB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
12
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
19
checked on Oct 12, 2024
Page view(s)
54
checked on Sep 7, 2022
Download(s)
42
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.