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Title: Thermal and chlorophyll-fluorescence imaging distinguish plant-pathogen interactions at an early stage
Authors: Chaerle, L
Hagenbeek, D
De Bruyne, E
VALCKE, Roland 
Van der Straeten, D
Issue Date: 2004
Source: PLANT AND CELL PHYSIOLOGY, 45(7). p. 887-896
Abstract: Different biotic stresses yield specific symptoms, owing to their distinct influence on a plant's physiological status. To monitor early changes in a plant's physiological status upon pathogen attack, chlorophyll fluorescence imaging (Chl-FI) and thermography, which respectively visualize photosynthetic efficiency and transpiration, were carried out in parallel for two fundamentally different plant-pathogen interactions. These non-destructive imaging techniques were able to visualize infections at an early stage, before damage appeared. Under growth-room conditions, a robotized set-up captured time series of visual, thermal and chlorophyll fluorescence images from infected regions on attached leaves. As a first symptom of the plant-virus interaction between resistant tobacco and tobacco mosaic virus (TMV), thermal imaging detected a local rise in temperature while Chi-FI monitored a co-localized increase in fluorescence intensity. Chl-FI also revealed pre-symptomatic high-intensity spots for the plant-fungus system sugar beet-Cercospora beticola. Concomitantly, spots of lower temperature were monitored with thermography, in marked contrast with our observations on TMV-infection in tobacco. Knowledge of disease signatures for different plant-pathogen interactions could allow early identification of emerging biotic stresses in crops, facilitating the containment of disease outbreaks. Presymptomatic monitoring clearly opens perspectives for quantitative screening for disease resistance, either on excised leaf pieces or attached leaves.
Notes: State Univ Ghent, Unite Hormone & Bioimaging, Dept Mol Genet, B-9000 Ghent, Belgium. Advanta SES Europe, Dept Biotechnol, B-3300 Tienen, Belgium. Limburgs Univ Ctr, Lab Mol & Phys Plant Physiol, B-3590 Diepenbeek, Belgium.Van der Straeten, D, State Univ Ghent, Unite Hormone & Bioimaging, Dept Mol Genet, KL Ledeganckstr 35, B-9000 Ghent,
Keywords: Cercospora beticola sacc; chlorophyll fluorescence imaging; hypersensitive response; plant-pathogen interaction; thermography; tobacco mosaic virus
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ISSN: 0032-0781
e-ISSN: 1471-9053
DOI: 10.1093/pcp/pch097
ISI #: 000223171700009
Category: A1
Type: Journal Contribution
Validations: ecoom 2005
Appears in Collections:Research publications

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