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http://hdl.handle.net/1942/22747
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DC Field | Value | Language |
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dc.contributor.author | Stancescu, Maria | - |
dc.contributor.author | Fedotova, Tatiana A. | - |
dc.contributor.author | HOOYBERGHS, Jef | - |
dc.contributor.author | Balaeff, Alexander | - |
dc.contributor.author | Kolpashchikov, Dmitry M. | - |
dc.date.accessioned | 2016-11-24T13:41:54Z | - |
dc.date.available | 2016-11-24T13:41:54Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 138(41), p. 13465-13468 | - |
dc.identifier.issn | 0002-7863 | - |
dc.identifier.uri | http://hdl.handle.net/1942/22747 | - |
dc.description.abstract | Detection of point mutations and single nucleotide polymorphisms in DNA and RNA has a growing importance in biology, biotechnology, and medicine. For the application at hand, hybridization assays are often used. Traditionally, they differentiate point mutations only at elevated temperatures (>40 degrees C) and in narrow intervals (Delta T = 1-10 degrees C). The current study demonstrates that a specially designed multistranded DNA probe can differentiate point mutations in the range of 5-40 degrees C. This unprecedentedly broad ambient-temperature range is enabled by a controlled combination of (i) nonequilibrium hybridization conditions and (ii) a mismatch-induced increase of equilibration time in respect to that of a fully matched complex, which we dub "kinetic inversion". | - |
dc.description.sponsorship | We thank Dr. Yulia V. Gerasimova for helpful discussions. D.M.K. was funded by NSF CCF 1423219 and NIAID R15AI10388001A1. J.H. received funding from the Research Fund - Flanders (FWO K226314N). A.B. was supported by the UCF startup funds. The computational time for the project was provided by the UCF STOKES cluster. | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.rights | (c) American Chemical Society | - |
dc.title | Nonequilibrium Hybridization Enables Discrimination of a Point Mutation within 5-40 degrees C | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 13468 | - |
dc.identifier.issue | 41 | - |
dc.identifier.spage | 13465 | - |
dc.identifier.volume | 138 | - |
local.format.pages | 4 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Stancescu, Maria; Fedotova, Tatiana A.; Kolpashchikov, Dmitry M.] Univ Cent Florida, Dept Chem, Orlando, FL 32816 USA. [Kolpashchikov, Dmitry M.] Univ Cent Florida, Natl Ctr Forens Sci, Orlando, FL 32816 USA. [Kolpashchikov, Dmitry M.] Univ Cent Florida, Burnett Sch Biomed Sci, Orlando, FL 32816 USA. [Hooyberghs, Jef] VITO, Flemish Inst Technol Res, Boeretang 200, B-2400 Mol, Belgium. [Hooyberghs, Jef] Hasselt Univ, Theoret Phys, Campus Diepenbeek,Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. [Balaeff, Alexander] NanoSci Technol Ctr, 12424 Res Pkwy,Suite 400, Orlando, FL 32826 USA. | - |
local.publisher.place | WASHINGTON | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1021/jacs.6b05628 | - |
dc.identifier.isi | 000385992100004 | - |
item.fulltext | With Fulltext | - |
item.accessRights | Open Access | - |
item.validation | ecoom 2017 | - |
item.contributor | Stancescu, Maria | - |
item.contributor | Fedotova, Tatiana A. | - |
item.contributor | HOOYBERGHS, Jef | - |
item.contributor | Balaeff, Alexander | - |
item.contributor | Kolpashchikov, Dmitry M. | - |
item.fullcitation | Stancescu, Maria; Fedotova, Tatiana A.; HOOYBERGHS, Jef; Balaeff, Alexander & Kolpashchikov, Dmitry M. (2016) Nonequilibrium Hybridization Enables Discrimination of a Point Mutation within 5-40 degrees C. In: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 138(41), p. 13465-13468. | - |
crisitem.journal.issn | 0002-7863 | - |
crisitem.journal.eissn | 1520-5126 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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JACS.pdf Restricted Access | Published version | 1.22 MB | Adobe PDF | View/Open Request a copy |
MS paper-1 revised_4.pdf | Peer-reviewed author version | 565.5 kB | Adobe PDF | View/Open |
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