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http://hdl.handle.net/1942/22747
Title: | Nonequilibrium Hybridization Enables Discrimination of a Point Mutation within 5-40 degrees C | Authors: | Stancescu, Maria Fedotova, Tatiana A. HOOYBERGHS, Jef Balaeff, Alexander Kolpashchikov, Dmitry M. |
Issue Date: | 2016 | Publisher: | AMER CHEMICAL SOC | Source: | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 138(41), p. 13465-13468 | Abstract: | Detection of point mutations and single nucleotide polymorphisms in DNA and RNA has a growing importance in biology, biotechnology, and medicine. For the application at hand, hybridization assays are often used. Traditionally, they differentiate point mutations only at elevated temperatures (>40 degrees C) and in narrow intervals (Delta T = 1-10 degrees C). The current study demonstrates that a specially designed multistranded DNA probe can differentiate point mutations in the range of 5-40 degrees C. This unprecedentedly broad ambient-temperature range is enabled by a controlled combination of (i) nonequilibrium hybridization conditions and (ii) a mismatch-induced increase of equilibration time in respect to that of a fully matched complex, which we dub "kinetic inversion". | Notes: | [Stancescu, Maria; Fedotova, Tatiana A.; Kolpashchikov, Dmitry M.] Univ Cent Florida, Dept Chem, Orlando, FL 32816 USA. [Kolpashchikov, Dmitry M.] Univ Cent Florida, Natl Ctr Forens Sci, Orlando, FL 32816 USA. [Kolpashchikov, Dmitry M.] Univ Cent Florida, Burnett Sch Biomed Sci, Orlando, FL 32816 USA. [Hooyberghs, Jef] VITO, Flemish Inst Technol Res, Boeretang 200, B-2400 Mol, Belgium. [Hooyberghs, Jef] Hasselt Univ, Theoret Phys, Campus Diepenbeek,Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. [Balaeff, Alexander] NanoSci Technol Ctr, 12424 Res Pkwy,Suite 400, Orlando, FL 32826 USA. | Document URI: | http://hdl.handle.net/1942/22747 | ISSN: | 0002-7863 | e-ISSN: | 1520-5126 | DOI: | 10.1021/jacs.6b05628 | ISI #: | 000385992100004 | Rights: | (c) American Chemical Society | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
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JACS.pdf Restricted Access | Published version | 1.22 MB | Adobe PDF | View/Open Request a copy |
MS paper-1 revised_4.pdf | Peer-reviewed author version | 565.5 kB | Adobe PDF | View/Open |
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