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|Title:||Sustained synchronized neuronal network activity in a human astrocyte co-culture system||Authors:||Kuijlaars, Jacobine
Detrez, Jan R.
De Vos, Winnok H.
Peeters, Pieter J.
|Issue Date:||2016||Publisher:||NATURE PUBLISHING GROUP||Source:||SCIENTIFIC REPORTS, 6 (Art N° 36529)||Abstract:||Impaired neuronal network function is a hallmark of neurodevelopmental and neurodegenerative disorders such as autism, schizophrenia, and Alzheimer's disease and is typically studied using genetically modified cellular and animal models. Weak predictive capacity and poor translational value of these models urge for better human derived in vitro models. The implementation of human induced pluripotent stem cells (hiPSCs) allows studying pathologies in differentiated disease-relevant and patient-derived neuronal cells. However, the differentiation process and growth conditions of hiPSC-derived neurons are non-trivial. In order to study neuronal network formation and (mal) function in a fully humanized system, we have established an in vitro co-culture model of hiPSC-derived cortical neurons and human primary astrocytes that recapitulates neuronal network synchronization and connectivity within three to four weeks after final plating. Live cell calcium imaging, electrophysiology and high content image analyses revealed an increased maturation of network functionality and synchronicity over time for co-cultures compared to neuronal monocultures. The cells express GABAergic and glutamatergic markers and respond to inhibitors of both neurotransmitter pathways in a functional assay. The combination of this co-culture model with quantitative imaging of network morphofunction is amenable to high throughput screening for lead discovery and drug optimization for neurological diseases.||Notes:||[Kuijlaars, Jacobine; Meert, Theo; Brone, Bert] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Oyelami, Tutu; Diels, Annick; Rohrbacher, Jutta; Versweyveld, Sofie; Meneghello, Giulia; Tuefferd, Marianne; Meert, Theo; Peeters, Pieter J.; Cik, Miroslav; Nuydens, Rony; Verheyen, An] Janssen Res & Dev, B-2340 Beerse, Belgium. [Verstraelen, Peter; Detrez, Jan R.; Verschuuren, Marlies; De Vos, Winnok H.] Univ Antwerp, Dept Vet Sci, B-2020 Antwerp, Belgium. [De Vos, Winnok H.] Univ Ghent, Dept Mol Biotechnol, B-9000 Ghent, Belgium.||Document URI:||http://hdl.handle.net/1942/22813||ISSN:||2045-2322||e-ISSN:||2045-2322||DOI:||10.1038/srep36529||ISI #:||000387043600001||Rights:||© The Author(s) 2016 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/||Category:||A1||Type:||Journal Contribution||Validations:||ecoom 2017|
|Appears in Collections:||Research publications|
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