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Title: Changes in chloroplast ultrastructure in Pssu-ipt tobacco during plant ontogeny
Authors: SYNKOVÁ, Helena
Pechova, R
VALCKE, Roland 
Issue Date: 2003
Source: PHOTOSYNTHETICA, 41(1). p. 117-126
Abstract: Changes in chloroplast ultrastructure and total content of endogenous cytokinins (CK) were studied during different phases of plant development in transgenic Pssu-ipt tobacco (Nicotiana tabacum L. cv. Petit Havana SR1). Permanent overproduction of CK was found in both rooted (SE) and grafted (G) Pssu-ipt plants in all phases of plant development with the peak in vegetative and flowering phase in the latter ones. No such a correlation was observed in SE on the contrary to control non-transgenic plants (SR1) and grafts (SRG), which showed also CK increase at juvenile and flowering phases. No significant differences in parameters of chloroplast ultrastructure, such as length of chloroplast, starch content, granum width, and number of thylakoids per granum, were proved between chloroplasts from young mature leaves of control and transgenic tobacco during plant ontogeny. Nevertheless, several anomalies in the ultrastructure of cell organelles were found in Pssu-ipt tobacco. Amoeboid shape of chloroplasts was often observed in connection with "tubular clusters" resembling peripheral reticulum. The distinct crystalline structures located in chloroplasts might be formed by LHC protein aggregates. Smaller crystals of unknown composition were found also in mitochondria. Numerous crystalline cores were present in peroxisomes. The alterations might be the result of imbalance of phytohormone content, degradation effect of CK overproduction, or the example of acclimation to permanent stress.
Notes: Acad Sci Czech Republ, Inst Expt Bot, CZ-16000 Prague 6, Czech Republic. Charles Univ, Fac Sci, Dept Plant Physiol, CZ-12844 Prague, Czech Republic. Limburgs Univ Centrum, Univ SBG, B-3500 Diepenbeek, Belgium.Synkovka, H, Acad Sci Czech Republ, Inst Expt Bot, Na Karlovce 1a, CZ-16000 Prague 6, Czech Republic.
Keywords: chloroplast ultrastructure; cytokinins; grafting; granum; leaf age; Nicotiana; peroxisome; phytohormones; thylakoid; transgenic plants
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ISSN: 0300-3604
e-ISSN: 1573-9058
DOI: 10.1023/A:1025829017298
ISI #: 000184725400017
Category: A1
Type: Journal Contribution
Validations: ecoom 2004
Appears in Collections:Research publications

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