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Title: Age-specific function of alpha 5 beta 1 integrin in microglial migration during early colonization of the developing mouse cortex
Authors: Smolders, Sophie M.T. 
Swinnen, Nina
Kessels, Sofie 
Arnauts, Kaline
Smolders, Silke 
Le Bras, Barbara
Rigo, Jean-Michel 
Legendre, Pascal
Brône, Bert 
Issue Date: 2017
Source: GLIA (New York, N.Y. : Print), 65(7), p. 1072-1088
Abstract: Microglia, the immune cells of the central nervous system, take part in brain development and homeostasis. They derive from primitive myeloid progenitors that originate in the yolk sac and colonize the brain mainly through intensive migration. During development, microglial migration speed declines which suggests that their interaction with the microenvironment changes. However, the matrix-cell interactions allowing dispersion within the parenchyma are unknown. Therefore, we aimed to better characterize the migration behavior and to assess the role of matrix-integrin interactions during microglial migration in the embryonic brain ex vivo. We focused on microglia-fibronectin interactions mediated through the fibronectin receptor alpha 5 beta 1 integrin because in vitro work indirectly suggested a role for this ligand-receptor pair. Using 2-photon time-lapse microscopy on acute ex vivo embryonic brain slices, we found that migration occurs in a saltatory pattern and is developmentally regulated. Most importantly, there is an age-specific function of the alpha 5 beta 1 integrin during microglial cortex colonization. At embryonic day (E) 13.5, alpha 5 beta 1 facilitates migration while from E15.5, it inhibits migration. These results indicate a developmentally regulated function of alpha 5 beta 1 integrin in microglial migration during colonization of the embryonic brain.
Keywords: blood vessels; cell adhesion molecules; cell migration inhibition; embryo; fibronectins
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ISSN: 0894-1491
e-ISSN: 1098-1136
DOI: 10.1002/glia.23145
ISI #: 000401345400005
Rights: (C) 2017 Wiley Periodicals
Category: A1
Type: Journal Contribution
Validations: ecoom 2018
Appears in Collections:Research publications

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