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Title: | Human papillomavirus genotype and viral load agreement between paired first-void urine and clinician-collected cervical samples | Authors: | Van Keer, Severien Tjalma, Wiebren A. A. Pattyn, Jade Biesmans, Samantha PIETERS, Zoe Van Ostade, Xaveer Ieven, Margareta Van Damme, Pierre Vorsters, Alex |
Issue Date: | 2018 | Source: | EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 37(5), p. 859-869 | Abstract: | The performance and acceptability of first-void urine as specimen for the detection of HPV DNA in a Belgian referral population was evaluated using an optimized sample collection and processing protocol. One hundred ten first-void urine and cervical samples were collected from 25- to 64-year-old women who were referred for colposcopy (January-November 2016). Paired samples were analyzed by the Riatol qPCR HPV genotyping assay. Acceptability data were gathered through questionnaires (NCT02714127). A higher high-risk HPV DNA prevalence was observed in first-void urine (n = 76/110) compared to cervical samples (n = 73/110), with HPV31 and HPV16/31 being most prevalent correspondingly. For both any and high-risk HPV DNA, good agreement was observed between paired samples (Cohen's Kappa of 0.660 (95% CI: 0.486-0.833) and 0.688 (95% CI: 0.542-0.835), respectively). In addition, significant positive correlations in HPV copies (per microliter of DNA extract) between paired samples were observed for HPV16 (r(s) = 0.670; FDR (false discovery rate)-adjusted p = 0.006), HPV18 (r(s) = 0.893; FDR-adjusted p = 0.031), HPV31 (r(s) = 0.527; FDR-adjusted p = 0.031), HPV53 (r(s) = 0.691; FDR-adjusted p = 0.017), and HPV68 (r(s) = 0.569; FDR-adjusted p = 0.031). First-void urine sampling using a first-void urine collection device was preferred over a clinician-collected cervical sample. And mostly, first-void urine sampling at home was favored over collection at the clinic or the general practitioner's office. First-void urine sampling is a highly preferred, non-invasive method that ensures good agreement in HPV DNA (copies) with reference cervical samples. It is particularly interesting as a screening technique to reach non-participants, and its clinical performance should be further evaluated. | Notes: | Van Keer, S (reprint author), Univ Antwerp, CEV, Vaccine & Infect Dis Inst VAXINFECTIO, Fac Med & Hlth Sci, Univ Pl 1, B-2610 Antwerp, Belgium. severien.vankeer@uantwerpen.be | Document URI: | http://hdl.handle.net/1942/26247 | ISSN: | 0934-9723 | e-ISSN: | 1435-4373 | DOI: | 10.1007/s10096-017-3179-1 | ISI #: | 000430981900007 | Rights: | Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2019 |
Appears in Collections: | Research publications |
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van keer 2.pdf | Published version | 922.76 kB | Adobe PDF | View/Open |
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