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Title: | Angiogenic Properties of 'Leukocyte- and Platelet-Rich Fibrin' | Authors: | RATAJCZAK, Jessica VANGANSEWINKEL, Tim GERVOIS, Pascal MERCKX, Greet HILKENS, Petra Quirynen, Marc LAMBRICHTS, Ivo BRONCKAERS, Annelies |
Issue Date: | 2018 | Source: | Scientific Reports, 8 (Art N° 14632) | Abstract: | Leukocyte- and Platelet-Rich Fibrin (L-PRF) is an autologous platelet concentrate, consisting of a fbrin matrix enriched with platelets, leukocytes and a plethora of cytokines and growth factors. Since L-PRF is produced bedside from whole blood without the use of an anti-coagulant, it is becoming a popular adjuvant in regenerative medicine. While other types of platelet concentrates have been described to stimulate blood vessel formation, little is known about the angiogenic capacities of L-PRF. Therefore, this study aimed to fully characterize the angiogenic potential of L-PRF. With an antibody array, the growth factors released by L-PRF were determined and high levels of CXC chemokine receptor 2 (CXCR-2) ligands and epidermal growth factor (EGF) were found. L-PRF induced in vitro key steps of the angiogenic process: endothelial proliferation, migration and tube formation. In addition, we could clearly demonstrate that L-PRF is able to induce blood vessel formation in vivo, the chorioallantoic membrane assay. In conclusion, we could demonstrate the angiogenic capacity of L-PRF both in vitro and in vivo, underlying the clinical potential of this easy-to-use platelet concentrate. | Notes: | Bronckaers, A (reprint author), Hasselt Univ, Fac Med & Life Sci, Biomed Res Inst, Dept Morphol, Diepenbeek, Belgium. annelies.bronckaers@uhasselt.be | Document URI: | http://hdl.handle.net/1942/27305 | ISSN: | 2045-2322 | e-ISSN: | 2045-2322 | DOI: | 10.1038/s41598-018-32936-8 | ISI #: | 000446034000033 | Rights: | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2019 |
Appears in Collections: | Research publications |
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s41598-018-32936-8.pdf | Published version | 1.59 MB | Adobe PDF | View/Open |
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