Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/27861
Title: | Hydration and Confinement Effects on Horse Heart Myoglobin Adsorption in Mesoporous TiO2 | Authors: | Loreto, Stefano Hafideddine, Zainab Cuypers, Bert CARLEER, Robert VAN DOORSLAER, Sabine Meynen, Vera |
Issue Date: | 2018 | Publisher: | AMER CHEMICAL SOC | Source: | Journal of Physical Chemistry C, 122(41), p. 23393-23404 | Abstract: | Despite the intensive research on protein adsorption in mesoporous materials, the effect of (de)hydration and confinement on the adsorbed protein's stability and activity is poorly understood. In this paper, we study the effect of differences in structural features (pore size) and drying time on the adsorption and structural stability of horse heart myoglobin (hhMb) on mesoporous titanium dioxide. Infrared spectroscopy (DRIFT) and thermal analysis (TGA) coupled to a quadrupole mass spectrometer (TGA-MS) were used to evaluate the impact of the confinement in different pores and hydration on the myoglobin secondary structure. Electron paramagnetic spectroscopy (EPR) was applied to identify the changes in the heme and its close surrounding. The peroxidase-like activity of myoglobin toward 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) in the presence of hydrogen peroxide allowed us to detect changes in the protein activity after adsorption in pores with different sizes and drying for different periods of time. The results show a clear effect of the pore size and drying time on the secondary structure of hhMb, which is confirmed by differences induced in the catalytic activity of the adsorbed proteins. Therefore, we recommend evaluating the effect of both hydration and confinement in future applications involving biomolecule adsorption in porous matrices. | Notes: | [Loreto, Stefano; Meynen, Vera] Univ Antwerp, Dept Chem, Lab Adsorpt & Catalysis LADCA, Univ Pl 1, B-2610 Antwerp, Belgium. [Hafideddine, Zainab; Cuypers, Bert; Van Doorslaer, Sabine] Univ Antwerp, Dept Phys, Lab Biophys & Biomed Phys BIMEF, Univ Pl 1, B-2610 Antwerp, Belgium. [Hafideddine, Zainab] Univ Antwerp, Dept Biomed Sci, Lab Proteinchem Prote & Epigenet Signaling PPES, Univ Pl 1, B-2610 Antwerp, Belgium. [Carleer, Robert] Univ Hasselt, Inst Mat Res IMO, Lab Appl & Analyt Chem TANC, Agoralaan, B-3590 Diepenbeek, Belgium. | Document URI: | http://hdl.handle.net/1942/27861 | ISSN: | 1932-7447 | e-ISSN: | 1932-7455 | DOI: | 10.1021/acs.jpcc.8b04408 | ISI #: | 000448087900018 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2019 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
acs.jpcc.8b04408.pdf Restricted Access | Published version | 1.71 MB | Adobe PDF | View/Open Request a copy |
J Phys Chem C.pdf | Peer-reviewed author version | 1.5 MB | Adobe PDF | View/Open |
WEB OF SCIENCETM
Citations
4
checked on Oct 13, 2024
Page view(s)
50
checked on Mar 23, 2022
Download(s)
70
checked on Mar 23, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.