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http://hdl.handle.net/1942/28141| Title: | A lineage CLOUD for neoblasts | Authors: | Tran, Thao Anh GENTILE, Luca |
Issue Date: | 2018 | Publisher: | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Source: | SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 87 (SI), p. 22-29 | Abstract: | Inplanarians,pluripotencycanbestudiedinvivointheadultanimal,makingtheseanimalsauniquemodelsystemwherepluripotency-basedregeneration(PBR)—anditstherapeuticpotential—canbeinves-tigated.Thisreviewfocusesonrecentfindingstobuildacloudmodeloffaterestrictionlikelihoodforplanarianstemandprogenitorcells.Recently,acomputationalapproachbasedonfunctionalandmolec-ularprofilingatthesinglecelllevelwasproposedforhumanhematopoieticstemcells.Basedondatageneratedbothinvivoandexvivo,wehypothesizedthatplanarianstemcellscouldacquiremultipledirec-tionlineagebiases,followinga“badlands”landscape.Insteadofadiscretetree-likehierarchy,wherethepotencyofstem/progenitorcellsreducesstepwise,weproposeaContinuumofLOw-primedUnDiffer-entiatedPlanarianStem/ProgenitorCells(CLOUD-PSPCs).Everysubclassofneoblast/progenitorcellsisacloudoflikelihood,asthesinglecelltranscriptomicsdataindicate.TheCLOUD-HSPCsconceptwassubstantiatedbyinvitrodatafromcellculture;therefore,toconfirmtheCLOUD-PSPCsmodel,thepla-nariancommunityneedstodevelopnewtools,likelivecelltracking.FuturestudieswillallowadeeperunderstandingofPBRinplanarian,andthepossibleimplicationsforregenerativetherapiesinhuman. | Notes: | Gentile, L (reprint author), Fraunhofer Inst Biomed Engn, Pluripotency & Regenerat Grp, Joseph von Fraunhofer Weg 1, D-66280 Sulzbach, Germany. mckind75@gmail.com | Keywords: | Neoblast;Potency state transitions;Stem cell heterogeneity;CLOUD;Badlands landscape;Live cell tracking | Document URI: | http://hdl.handle.net/1942/28141 | ISSN: | 1084-9521 | e-ISSN: | 1096-3634 | DOI: | 10.1016/j.semcdb.2018.04.012 | ISI #: | 000458768200004 | Rights: | 2018 Elsevier Ltd. All rights reserved | Category: | A1 | Type: | Journal Contribution |
| Appears in Collections: | Research publications |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Trann_Gentile_CLOUD_SCDB 1.pdf | Peer-reviewed author version | 3.88 MB | Adobe PDF | View/Open |
| A lineage cloud.pdf Restricted Access | Published version | 18.63 MB | Adobe PDF | View/Open Request a copy |
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