Please use this identifier to cite or link to this item:
Title: The Sycamore Maple Bacterial Culture Collection From a TNT Polluted Site Shows Novel Plant-Growth Promoting and Explosives Degrading Bacteria
Authors: THIJS, Sofie 
SILLEN, Wouter 
TRUYENS, Sascha 
van Hamme, Jonathan
van Dillewijn, Pieter
SAMYN, Pieter 
CARLEER, Robert 
Issue Date: 2018
Source: Frontiers in Plant Science, 9 (Art N° 1134)
Abstract: Military activities have worldwide introduced toxic explosives into the environment with considerable effects on soil and plant-associated microbiota. Fortunately, these microorganisms, and their collective metabolic activities, can be harnessed for site restoration via in situ phytoremediation. We characterized the bacterial communities inhabiting the bulk soil and rhizosphere of sycamore maple (Acer pseudoplatanus) in two chronically 2,4,6-trinitrotoluene (TNT) polluted soils. Three hundred strains were isolated, purified and characterized, a majority of which showed multiple plant growth promoting (PGP) traits. Several isolates showed high nitroreductase enzyme activity and concurrent TNT-transformation. A 12-member bacterial consortium, comprising selected TNT-detoxifying and rhizobacterial strains, significantly enhanced TNT removal from soil compared to non-inoculated plants, increased root and shoot weight, and the plants were less stressed than the un-inoculated plants as estimated by the responses of antioxidative enzymes. The sycamore maple tree (SYCAM) culture collection is a significant resource of plant-associated strains with multiple PGP and catalytic properties, available for further genetic and phenotypic discovery and use in field applications.
Keywords: plant-associated bacteria; Acer pseudoplatanus; plant-growth-promoting-bacteria; TNT degradation; culture collections
Document URI:
ISSN: 1664-462X
e-ISSN: 1664-462X
DOI: 10.3389/fpls.2018.01134
ISI #: 000440679500001
Rights: Copyright © 2018 Thijs, Sillen, Truyens, Beckers, van Hamme, van Dillewijn, Samyn, Carleer, Weyens and Vangronsveld. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Category: A1
Type: Journal Contribution
Validations: ecoom 2019
Appears in Collections:Research publications

Files in This Item:
File Description SizeFormat 
fpls-09-01134.pdfPublished version2.54 MBAdobe PDFView/Open
Show full item record


checked on Sep 2, 2020


checked on May 13, 2022

Page view(s)

checked on Mar 23, 2022


checked on Mar 23, 2022

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.