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Title: | Preconditioning of Human Dental Pulp Stem Cells with Leukocyte- and Platelet-Rich Fibrin-Derived Factors Does Not Enhance Their Neuroregenerative Effect | Authors: | GERVOIS, Pascal RATAJCZAK, Jessica WOLFS, Esther VANGANSEWINKEL, Tim DILLEN, Yorg MERCKX, Greet BRONCKAERS, Annelies LAMBRICHTS, Ivo |
Issue Date: | 2019 | Source: | Stem cells international, (Art N° 8589149) | Abstract: | Pathologies of the central nervous system are characterized by loss of brain tissue and neuronal function which cannot be adequately restored by endogenous repair processes. This stresses the need for novel treatment options such as cell-based therapies that are able to restore damaged tissue or stimulate repair. This study investigated the neuroregenerative potential of the conditioned medium of human dental pulp stem cells (CM-hDPSCs) on neural stem cell (NSC) proliferation and migration as well as on neurite outgrowth of primary cortical neurons (pCNs). Additionally, the effect of leukocyte- and platelet-rich fibrin (L-PRF) priming on the neuroregenerative potential of the hDPSC secretome on NSCs and pCNs was evaluated. L-PRF contains factors that enhance stem cell-induced regeneration, but its effect on hDPSC-mediated neuroregeneration is unknown. This study demonstrated that CM-hDPSCs enhanced neuritogenesis. Moreover, CM-hDPSCs had a chemoattractant effect on NSCs. Although priming hDPSCs with L-PRF increased brain-derived neurotrophic factor secretion, no additional effects on the paracrine-mediated repair mechanisms were observed. These data support the neuroregenerative potential of hDPSCs, and although priming had no additional effect, the potential of L-PRF-primed hDPSCs on distinct regenerative mechanisms remains to be clarified. | Notes: | Part of this work has been previously presented as a poster presentation and abstract on the 4th Annual Meeting of the Belgian Society for Stem Cell Research on November 24th, 2017, Liège, Belgium. | Document URI: | http://hdl.handle.net/1942/28543 | Link to publication/dataset: | https://www.hindawi.com/journals/sci/2019/8589149/cta/ | ISSN: | 1687-966X | e-ISSN: | 1687-9678 | DOI: | 10.1155/2019/8589149 | ISI #: | 000465341200001 | Rights: | 2019 Pascal Gervois et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
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Gervois 2019 Preconditioning of hDPSCs with LPRF does not enhance their neuroregenerative effect.pdf | Published version | 8.58 MB | Adobe PDF | View/Open |
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