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Title: | Low-dose radiations derived from cone-beam CT induce transient DNA damage and persistent inflammatory reactions in stem cells from deciduous teeth | Authors: | Virag, Piroska Hedesiu, Mihaela Soritau, Olga Perde-Schrepler, Maria Brie, Ioana Pall, Emoke Fischer-Fodor, Eva Bogdan, Toredana Lucaciu, Ondine BELMANS, Niels Moreels, Madan Salmon, Benjamin Jacobs, Reinhilde |
Issue Date: | 2019 | Publisher: | BRITISH INST RADIOLOGY | Source: | DENTOMAXILLOFACIAL RADIOLOGY, 48(1) (Art N° 20170462) | Abstract: | Objectives: Cone-beam CT (CBCT), a radiographic tool for diagnosis, treatment, and follow-up in dental practice, was introduced also in pediatric radiology, especially orthodontics. Such patients subjected to repetitive X-rays examinations may receive substantial levels of radiation doses. Ionizing radiation (IR), a recognized carcinogenic factor causing DNA double-strand breaks (DSBs) could be harmful to undifferentiated cells such as dental pulp stem cells (DPSCs) since inaccurately repaired or unrepaired DSBs may lead to malignant transformation. The H2AX and MRE11 proteins generated following DSBs formation and pro-inflammatory cytokines (CKs) secreted after irradiation are relevant candidates to monitor the cellular responses induced by CBCT. Methods: DPSCs were extracted from human exfoliated deciduous teeth and their phenotype was assessed by immunocytochemistry and flow-cytometry. Cells were exposed to IR doses: 5.4-107.7 mGy, corresponding to 0.5-8 consecutive skull exposures, respectively. H2AX and MRE11 were detected in whole cells, while IL-1 alpha, IL-6, IL-8, TNF alpha in supernatants, using enzyme-linked immunosorbent assay (ELISA) at different time points after exposure. Results: The phosphorylation level of H2AX in DPSCs increased considerably at 0.5 h after exposure (p < 0.001 for 3, 5, 8 skull exposures and p < 0.05 for 1 skull exposure, respectively). MRE11 response could only be detected for the highest IR dose (p < 0.001) in the same interval. CKs secretion increased upon CBCT exposure according to doses and time. Conclusions: The DPSCs exposure to CBCT induces transient DNA damage and persistent inflammatory reaction in DPSCs drawing the attention on the potential risks of IR exposures and on the importance of dose monitoring in pediatric population. | Notes: | [Virag, Piroska; Soritau, Olga; Perde-Schrepler, Maria; Brie, Ioana; Fischer-Fodor, Eva] Oncol Inst Prof Dr Ion Chiricuta, Lab Radiotherapy Radiobiol & Tumor Biol, Cluj Napoca, Romania. [Hedesiu, Mihaela; Lucaciu, Ondine] Iuliu Hatieganu Univ Med & Pharm, Dept Oral & Maxillofacial Radiol, Cluj Napoca, Romania. [Pall, Emoke] Univ Agr Sci & Vet Med, Cluj Napoca, Romania. [Fischer-Fodor, Eva] Iuliu Hatieganu Univ Med & Pharm, Medfuture Res Ctr Adv Med, Cluj Napoca, Romania. [Bogdan, Toredana] Natl Inst Publ Hlth, Reg Ctr Publ Hlth Cluj Napoca, Radiat Hyg Dept, Cluj Napoca, Romania. [Belmans, Niels] Hasselt Univ, Fac Med & Life Sci, Biomed Res Inst, Hasselt, Belgium. [Belmans, Niels; Moreels, Madan] SCK CEN, Belgian Nucl Res Ctr, Radiobiol Unit, Interdisciplinary Biosci,Inst Environm Hlth & Saf, Boeretang, Belgium. [Salmon, Benjamin] Paris Descartes Univ, Dent Sch, Orofacial Pathol Imaging & Biotherapies, EA2496,Sorbonne Paris Cite, Paris, France. [Salmon, Benjamin] Nord Val de Seine Hosp Bretonneau, AP HP, Dept Odontol, Paris, France. [Jacobs, Reinhilde] Univ Leuven, Dept Imaging & Pathol, OMFS IMPATH Res Grp, Leuven, Belgium. | Keywords: | CBCT; H2AX; MRE11; inflammatory cytokines | Document URI: | http://hdl.handle.net/1942/28551 | ISSN: | 0250-832X | e-ISSN: | 1476-542X | DOI: | 10.1259/dmfr.20170462 | ISI #: | 000453231900003 | Rights: | 2019 The Authors. Published by the British Institute of Radiology | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
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virag 1.pdf Restricted Access | Published version | 1.9 MB | Adobe PDF | View/Open Request a copy |
Low-dose radiations derived from cone-beam CT induce transient DNA damage and persistent inflammatory reactions in stem cells from deciduous teeth.pdf | Peer-reviewed author version | 1.36 MB | Adobe PDF | View/Open |
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