Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/28624
Title: Microfluidic Diamond Biosensor Using NV Centre Charge State Detection
Authors: Krecmarova, Marie
VANDENRYT, Thijs 
GULKA, Michal 
BOURGEOIS, Emilie 
Fekete, Ladislav
Hubik, Pavel
THOELEN, Ronald 
MORTET, Vincent 
NESLADEK, Milos 
Issue Date: 2018
Publisher: Springer Singapore
Source: Sukupova, Lucie; Lacković, Igor; Ibbott, Geoffrey S.; Lhotska, Lenka; Lhotska, L Sukupova (Ed.). World Congress on Medical Physics and Biomedical Engineering 2018, Springer Singapore, p. 27 -31
Series/Report: IFMBE Proceedings
Series/Report no.: 68/3
Abstract: In this work we develop DNA sensors that are based on charge switching in colour centres in diamond. The presented method allows the combination of luminescence sensor and electrochemical sensor working on the principle of electrochemical impedance spectroscopy (EIS). The sensor employs specifically designed diamond structures grown by the means of chemical Vapour deposition (CVD). This diamond structure consists of highly boron doped diamond electrode on which an intrinsic diamond layer is deposited. This intrinsic layer is about 15 nm thick and it contains NV colour centres. The device is then embedded in polydimethylsiloxane (PDMS) microfluidic flow cell and covered by a transparent indium tin oxide (ITO) coated electrode. The switching of the NV centre charge state as a response, on diamond surface termination, is crucial tool for the sensitive charged molecules sensing. First we demonstrated high sensitivity of the near surface NV centres on a diamond biosensor surface charge termination. The measured data are supported by band bending modelling. Negative O- terminated surface results in a preferable NV centre charge state of NV0 or NV−, whereas positive H- terminated surface leads to mostly non-PL NV+ charge state. By this principle any charged molecule, such as polymer on DNA, can be detected by a customized surface functionalization. Functionality of the microfluidic diamond device is also verified by the EIS.
Notes: [Krecmarova, Marie; Gulka, Michal; Mortet, Vincent; Nesladek, Milos] Czech Tech Univ, Fac Biomed Engn, Nam Sitna 3105, Kladno 27201, Czech Republic. [Vandenryt, Thijs; Gulka, Michal; Bourgeois, Emilie; Thoelen, Ronald] Univ Hasselt, Inst Mat Res, Mat Phys Div, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. [Vandenryt, Thijs; Bourgeois, Emilie; Thoelen, Ronald] IMEC, IMOMEC Div, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. [Fekete, Ladislav; Hubik, Pavel; Mortet, Vincent] Acad Sci Czech Republ VVI, Inst Phys, Slovance 1999-2, Prague 18221 8, Czech Republic.
Keywords: Nitrogen vacancy;Diamond;Microfluidics
Document URI: http://hdl.handle.net/1942/30079
http://hdl.handle.net/1942/28624
DOI: 10.1007/978-981-10-9023-3_6
ISI #: 000449744300006
Rights: Springer Nature Singapore Pte Ltd. 2019
Category: C1
Type: Proceedings Paper
Validations: ecoom 2019
Appears in Collections:Research publications

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