Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/28624
Title: | Microfluidic Diamond Biosensor Using NV Centre Charge State Detection | Authors: | Krecmarova, Marie VANDENRYT, Thijs GULKA, Michal BOURGEOIS, Emilie Fekete, Ladislav Hubik, Pavel THOELEN, Ronald MORTET, Vincent NESLADEK, Milos |
Issue Date: | 2018 | Publisher: | Springer Singapore | Source: | Sukupova, Lucie; Lacković, Igor; Ibbott, Geoffrey S.; Lhotska, Lenka; Lhotska, L Sukupova (Ed.). World Congress on Medical Physics and Biomedical Engineering 2018, Springer Singapore, p. 27 -31 | Series/Report: | IFMBE Proceedings | Series/Report no.: | 68/3 | Abstract: | In this work we develop DNA sensors that are based on charge switching in colour centres in diamond. The presented method allows the combination of luminescence sensor and electrochemical sensor working on the principle of electrochemical impedance spectroscopy (EIS). The sensor employs specifically designed diamond structures grown by the means of chemical Vapour deposition (CVD). This diamond structure consists of highly boron doped diamond electrode on which an intrinsic diamond layer is deposited. This intrinsic layer is about 15 nm thick and it contains NV colour centres. The device is then embedded in polydimethylsiloxane (PDMS) microfluidic flow cell and covered by a transparent indium tin oxide (ITO) coated electrode. The switching of the NV centre charge state as a response, on diamond surface termination, is crucial tool for the sensitive charged molecules sensing. First we demonstrated high sensitivity of the near surface NV centres on a diamond biosensor surface charge termination. The measured data are supported by band bending modelling. Negative O- terminated surface results in a preferable NV centre charge state of NV0 or NV−, whereas positive H- terminated surface leads to mostly non-PL NV+ charge state. By this principle any charged molecule, such as polymer on DNA, can be detected by a customized surface functionalization. Functionality of the microfluidic diamond device is also verified by the EIS. | Notes: | [Krecmarova, Marie; Gulka, Michal; Mortet, Vincent; Nesladek, Milos] Czech Tech Univ, Fac Biomed Engn, Nam Sitna 3105, Kladno 27201, Czech Republic. [Vandenryt, Thijs; Gulka, Michal; Bourgeois, Emilie; Thoelen, Ronald] Univ Hasselt, Inst Mat Res, Mat Phys Div, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. [Vandenryt, Thijs; Bourgeois, Emilie; Thoelen, Ronald] IMEC, IMOMEC Div, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. [Fekete, Ladislav; Hubik, Pavel; Mortet, Vincent] Acad Sci Czech Republ VVI, Inst Phys, Slovance 1999-2, Prague 18221 8, Czech Republic. | Keywords: | Nitrogen vacancy;Diamond;Microfluidics | Document URI: | http://hdl.handle.net/1942/30079 http://hdl.handle.net/1942/28624 |
DOI: | 10.1007/978-981-10-9023-3_6 | ISI #: | 000449744300006 | Rights: | Springer Nature Singapore Pte Ltd. 2019 | Category: | C1 | Type: | Proceedings Paper | Validations: | ecoom 2019 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
microfluidic.pdf Restricted Access | Published version | 451.48 kB | Adobe PDF | View/Open Request a copy |
IUPESM2018_508_final_v2 (1).pdf | Peer-reviewed author version | 357.28 kB | Adobe PDF | View/Open |
WEB OF SCIENCETM
Citations
1
checked on Oct 12, 2024
Page view(s)
150
checked on Sep 7, 2022
Download(s)
140
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.