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Title: Role of nanoparticle size and sialic acids in the distinct time-evolution profiles of nanoparticle uptake in hematopoietic progenitor cells and monocytes
Authors: WATHIONG, Bart 
Jacobs, An
GERVOIS, Pascal 
AMELOOT, Marcel 
Nelissen, Inge
Issue Date: 2019
Publisher: BMC
Source: Journal of nanobiotechnology, 17 (Art N° 62)
Abstract: BackgroundHuman hematopoietic progenitor cells (HPCs) are important for cell therapy in cancer and tissue regeneration. In vitro studies have shown a transient association of 40nm polystyrene nanoparticles (PS NPs) with these cells, which is of interest for intelligent design and application of NPs in HPC-based regenerative protocols. In this study, we aimed to investigate the involvement of nanoparticles' size and membrane-attached glycan molecules in the interaction of HPCs with PS NPs, and compared it with monocytes. Human cord blood-derived HPCs and THP-1 cells were exposed to fluorescently labelled, carboxylated PS NPs of 40, 100 and 200nm. Time-dependent nanoparticle membrane association and/or uptake was observed by measuring fluorescence intensity of exposed cells at short time intervals using flow cytometry. By pretreating the cells with neuraminidase, we studied the possible effect of membrane-associated sialic acids in the interaction with NPs. Confocal microscopy was used to visualize the cell-specific character of the NP association.ResultsConfocal images revealed that the majority of PS NPs was initially observed to be retained at the outer membrane of HPCs, while the same NPs showed immediate internalization by THP-1 monocytic cells. After prolonged exposure up to 4h, PS NPs were also observed to enter the HPCs' intracellular compartment. Cell-specific time courses of NP association with HPCs and THP-1 cells remained persistent after cells were enzymatically treated with neuraminidase, but significantly increased levels of NP association could be observed, suggesting a role for membrane-associated sialic acids in this process.ConclusionsWe conclude that the terminal membrane-associated sialic acids contribute to the NP retention at the outer cell membrane of HPCs. This retention behavior is a unique characteristic of the HPCs and is independent of NP size.
Notes: [Wathiong, Bart; Deville, Sarah; Jacobs, An; Hooyberghs, Jef; Nelissen, Inge] Flemish Inst Technol Res VITO, Hlth Dept, Boeretang 200, B-2400 Mol, Belgium. [Smisdom, Nick; Gervois, Pascal; Lambrichts, Ivo; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst BIOMED, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Hooyberghs, Jef] Hasselt Univ, Theoret Phys, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium.
Keywords: Nanoparticles; Hematopoietic progenitor cells; Monocytes; Flow cytometry; Confocal imaging; Proteoglycans;Biotechnology & Applied Microbiology; Nanoscience & Nanotechnology
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e-ISSN: 1477-3155
DOI: 10.1186/s12951-019-0495-x
ISI #: 000468053400001
Rights: The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Category: A1
Type: Journal Contribution
Validations: ecoom 2020
Appears in Collections:Research publications

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