Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/29645
Title: | Altered Gene Expression in Dioxin-Like and Non-Dioxin-Like PCB Exposed Peripheral Blood Mononuclear Cells | Authors: | Leijs, Marike M. Gan, Lin DE BOEVER, Patrick Esser, Andre Amann, Philipp M. Ziegler, Patrick Fietkau, Katharina Schettgen, Thomas Kraus, Thomas Merk, Hans F. Baron, Jens M. |
Issue Date: | 2019 | Publisher: | MDPI | Source: | INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 16(12) (Art N° 2090) | Abstract: | Polychlorinated biphenyls (PCBs) are well known carcinogenic persistent environmental pollutants and endocrine disruptors. Our aim was to identify the possible dysregulation of genes in PCB exposed peripheral blood mononuclear cells (PBMCs) in order to give more insight into the differential pathophysiological effects of PCB congeners and mixtures, with an emphasis on immunological effects and oxidative stress. The PBMCs of a healthy volunteer (male, 56 years old) were exposed to a mixture of dioxin-like (DL)-PCBs (PCB 77, 81, 105, 114, 118, 123, 126, 156, 157, 167, 169, and 189, 250 mu g/L resp.) or non-dioxin-like (NDL)-PCBs (PCB 28, 52, 101, 138, 153, 180, 250 mu g/L resp.) or single PCB congener (no. 28, 138, 153, 180, 250 mu g/L resp.). After an incubation period of 24 h, a microarray gene expression screening was performed, and the results were compared to gene expression in control samples (PBMCs treated with the vehicle iso-octane). Treatment of PBMCs with the DL-PCB mixture resulted in the largest number of differentially regulated genes (181 upregulated genes >2-fold, 173 downregulated >2-fold). Treatment with the NDL-PCB mix resulted in 32 upregulated genes >2-fold and 12 downregulated genes >2-fold. A gene set enrichment analysis (GSEA) on DL-PCB treated PBMCs resulted in an upregulation of 125 gene sets and a downregulation of 76 gene sets. Predominantly downregulated gene sets were involved in immunological pathways (such as response to virus, innate immune response, defense response). An upregulation of pathways related to oxidative stress could be observed for all PCB congeners except PCB-28; the latter congener dysregulated the least number of genes. Our experiment augments the information known about immunological and cellular stress responses following DL-as well as NDL-PCB exposure and provides new information on PCB 28. Further studies should be performed to evaluate how disruption of these pathways contributes to the development of autoimmune diseases and cancer. | Notes: | [Leijs, Marike M.; Amann, Philipp M.; Fietkau, Katharina; Merk, Hans F.; Baron, Jens M.] Rhein Westfal TH Aachen, Dept Dermatol & Allergol, D-52074 Aachen, Germany. [Gan, Lin] Rhein Westfal TH Aachen, IZKF, D-52074 Aachen, Germany. [De Boever, Patrick] Flemish Inst Technol Res VITO, Hlth Unit, B-2400 Mol, Belgium. [De Boever, Patrick] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. [Esser, Andre; Ziegler, Patrick; Schettgen, Thomas; Kraus, Thomas] Rhein Westfal TH Aachen, Inst Occupat Social & Environm Med, D-52074 Aachen, Germany. [Amann, Philipp M.] SLK Hosp Heilbronn, Dept Dermatol, D-74078 Heilbronn, Germany. | Keywords: | polychlorinated biphenyls; PBMC; gene regulation; PCB exposure; AHR; oxidative stress;polychlorinated biphenyls; PBMC; gene regulation; PCB exposure; AHR; oxidative stress | Document URI: | http://hdl.handle.net/1942/29645 | ISSN: | 1661-7827 | e-ISSN: | 1660-4601 | DOI: | 10.3390/ijerph16122090 | ISI #: | 000473750500024 | Rights: | 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (http://creativecommons.org/licenses/by/4.0/) | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
leijs 1.pdf | Published version | 1.03 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
1
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
12
checked on May 1, 2024
Page view(s)
88
checked on May 30, 2022
Download(s)
124
checked on May 30, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.