Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/29701
Title: | Conformational Changes as Driving Force for Phase Recognition: The Case of Laurdan | Authors: | Osella, Silvio SMISDOM, Nick AMELOOT, Marcel KNIPPENBERG, Stefan |
Issue Date: | 2019 | Publisher: | AMER CHEMICAL SOC | Source: | LANGMUIR, 35(35), p. 11471-11481 | Abstract: | The development of a universal probe to assess the phase of a lipid membrane is one of the most ambitious goals for fluorescence spectroscopy. The ability of a well-known molecule as Laurdan to reach this aim is here exploited as the behavior of the probe is fully characterized in a dipalmitoyl-phosphatidylcholine (DPPC) solid gel (So) phase by means of molecular dynamics simulations. Laurdan can take two conformations, depending on whether the carbonyl oxygen points toward the beta-position of the naphthalene core (Conf-I) or to the alpha-position (Conf-II). We observe that Conf-I has an elongated form in this environment, whereas Conf-II takes an L-shape. Interestingly, our theoretical calculations show that these two conformations behave in an opposite way from what is reported in the literature for a DPPC membrane in a liquid disordered (Ld) phase, where Conf-I assumes an L-shape and Conf-II is elongated. Moreover, our results show that in DPPC (So) no intermixing between the conformations is present, whereas it has been seen in a fluid environment such as DOPC (Ld). Through a careful analysis of angle distributions and by means of the rotational autocorrelation function, we predict that the two conformers of Laurdan behave differently in different membrane environments. | Notes: | [Osella, Silvio] Univ Warsaw, Ctr New Technol, Chem & Biol Syst Simulat Lab, Banacha 2C, PL-02097 Warsaw, Poland. [Smisdom, Nick; Ameloot, Marcel; Knippenberg, Stefan] Hasselt Univ, Biomed Res Inst, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Knippenberg, Stefan] Royal Inst Technol, Sch Biotechnol, Dept Theoret Chem & Biol, SE-10691 Stockholm, Sweden. [Knippenberg, Stefan] Palacky Univ, Fac Sci, Dept Phys Chem, RCPTM, Olomouc 77146, Czech Republic. | Document URI: | http://hdl.handle.net/1942/29701 | ISSN: | 0743-7463 | e-ISSN: | 1520-5827 | DOI: | 10.1021/acs.langmuir.9b01840 | ISI #: | 000484644000027 | Rights: | 2019 American Chemical Society | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
osella 1.pdf Restricted Access | Published version | 4.5 MB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
2
checked on Sep 5, 2020
WEB OF SCIENCETM
Citations
23
checked on Oct 14, 2024
Page view(s)
146
checked on Jun 17, 2022
Download(s)
126
checked on Jun 17, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.