Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/30659
Title: | Elevated expression of both mRNA and protein levels of IL-17A in sputum of stable Cystic Fibrosis patients | Authors: | Decraene, A WIDYASTUTI, Anna Kasran, A. De Boeck, K. Bullens, D.M. Dupont, L.J. |
Issue Date: | 2010 | Publisher: | BIOMED CENTRAL LTD |
Source: | Respiratory Research, 11 (1) , p. 177 (Art N° 177) | Abstract: | Background: T helper 17 (Th17) cells can recruit neutrophils to inflammatory sites through production of IL-17, which induces chemokine release. IL-23 is an important inducer of IL-17 and IL-22 production. Our aim was to study the role of Th17 cells in cystic fibrosis (CF) lung disease by measuring IL-17 protein and mRNA levels and IL-22 and IL-23 mRNA in sputum of clinically stable CF patients and by comparing these levels with healthy controls.Methods: Sputum induction was performed in adult CF patients outside of an exacerbation and healthy control subjects. IL-17A protein levels were measured in supernatants with cytometric bead array (CBA) and RNA was isolated and quantitative RT-PCR was performed for IL-17A, IL-22 and IL-23.Results: We found significantly higher levels of IL-17A protein and mRNA levels (both: p < 0.0001) and IL-23 mRNA levels (p < 0.0001) in the sputum of CF group as compared to controls. We found very low levels of IL-22 mRNA in the CF group. The levels of IL-17 and IL-23 mRNA were higher in patients chronically infected with Pseudomonas aeruginosa (P. aeruginosa) as compared to those who were not chronically infected with P. aeruginosa. The presence of Staphylococcus aureus (S. aureus) on sputum did not affect the IL-17 or IL-23 levels. There was no correlation between IL-17 or IL-23 levels and FEV1 nor sputum neutrophilia.Conclusion: The elevated levels of IL-17 and IL-23 might indicate that Th17 cells are implicated in the persistent neutrophil infiltration in CF lung disease and chronic infection with P. aeruginosa. | Keywords: | Adult;Cystic Fibrosis;Female;Humans;Interleukin-17;Interleukin-23;Male;RNA, Messenger;Sputum;Up-Regulation | Document URI: | http://hdl.handle.net/1942/30659 | Link to publication/dataset: | http://www.scopus.com/inward/record.url?eid=2-s2.0-79551592779&partnerID=MN8TOARS | DOI: | 10.1186/1465-9921-11-177 | ISI #: | WOS:000286117600001 | Rights: | © 2010 Decraene et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
decraene.pdf | Published version | 352.85 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
62
checked on Sep 2, 2020
WEB OF SCIENCETM
Citations
77
checked on Apr 22, 2024
Page view(s)
20
checked on Sep 7, 2022
Download(s)
8
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.