Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/30686
Title: Heterogeneity of chronic lung allograft dysfunction: Insights from protein expression in broncho alveolar lavage
Authors: Verleden, S.E.
Vos, R.
Mertens, V.
Willems-Widyastuti, A.
De Vleeschauwer, S.I.
DUPONT, Tim 
Verleden, G.M.
Van Raemdonck, D.E.
Vanaudenaerde, B.M.
Issue Date: 2011
Publisher: ELSEVIER SCIENCE INC
Source: Journal of Heart and Lung Transplantation, Journal of Heart and Lung Transplantation, 30 (6) , p. 667-673 -673
Abstract: BACKGROUND: Chronic lung allograft dysfunction (CLAD) remains a major risk factor for death after lung transplantation. Previous data suggested that within CLAD at least 2 phenotypes are present: a neutrophilic type (nCLAD or neutrophilic reversible allograft dysfunction [NRAD]), reversible with azithromycin therapy, vs a low neutrophilic type, non-responsive to azithromycin (fibrotic bronchiolitis obliterans syndrome [fBOS]). We aimed to further characterize this dichotomy by measuring multiple proteins in the bronchoalveolar lavage (BAL) fluid of 28 lung recipients.METHODS: Patients were retrospectively subdivided by the absence or presence of CLAD and subsequently by their response to azithromycin, resulting in 3 groups: 10 stable, 9 responsive (nCLAD/NRAD), and 9 non-responsive (fBOS). Enzyme-linked immunosorbent assay was used to measure 32 different proteins.RESULTS: Protein variations were predominantly present in the nCLAD/NRAD group, whereas no differences were observed in the fBOS group compared with control. MCP-1 (p < 0.01), RANTES (p < 0.05), IL-beta (p < 0.01), IL-8 (p < 0.01), TIMP-1 (p < 0.01), MMP-8 (p < 0.01), MMP-9 (p < 0.01), HGF (p < 0.001), MPO (p < 0.01), and bile acid (p < 0.05) concentrations were upregulated in nCLAD/NRAD compared with fBOS, whereas PDGF-AA (p < 0.05) was downregulated.CONCLUSIONS: These data provide further evidence that within CLAD there is a heterogeneity of phenotypes with different mechanisms involved. Further investigation is warranted to unravel the pathophysiology of both phenotypes. J Heart Lung Transplant 2011;30:667-73 (C) 2011 International Society for Heart and Lung Transplantation. All rights reserved.
Keywords: bronchiolitis obliterans syndrome;chronic lung allograft dysfunction;lung transplantation;neutrophilic reversible allograft dysfunction;bronchoalveolar lavage;lung rejection
Document URI: http://hdl.handle.net/1942/30686
Link to publication/dataset: http://www.scopus.com/inward/record.url?eid=2-s2.0-79955877424&partnerID=MN8TOARS
DOI: 10.1016/j.healun.2010.12.008
ISI #: WOS:000290834600009
Category: A1
Type: Journal Contribution
Appears in Collections:Research publications

Show full item record

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.