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Title: | Intracellular and luminal pH measurements of Malpighian tubules of the mosquito Aedes aegypti: the effects of cAMP | Authors: | Petzel, DH PIROTTE, Patrick VAN KERKHOVE, Emmy |
Issue Date: | 1999 | Publisher: | PERGAMON-ELSEVIER SCIENCE LTD | Source: | JOURNAL OF INSECT PHYSIOLOGY, 45(11). p. 973-982 | Abstract: | In order to understand the critical role that hydrogen ions play in fluid secretion in Malpighian tubules, intracellular and luminal pH and K+ measurements were performed in isolated Malpighian tubules of the yellow fever mosquito (Aedes aegypti). The intracellular pH was 7.03+/-0.05 (n=15 Malpighian tubules (MT)) and the luminal pH was 7.19+/-0.09 (n=99 MT) when bathed in saline at a pH of 7.0, The lumen potential is positive, thus net proton secretion into the lumen is active. The intracellular and the luminal K+ concentrations were 75+/-9 mM (n=15) and 102+/-13 mM (n=9 MT) respectively. Cyclic AMP analogues accelerated fluid secretion and at the same time acidified the cell without affecting the luminal pH. Both effects were abolished by an isomer of adenosine-3',5' cyclic monophosphothioate (cAMPS), the Rp-cAMPS, known to inhibit protein kinase A. The results suggest that in the presence of cAMP the properties of the cation/H+ exchanger are affected and that this may be a result of phosphorylation of a Na+/2H+ antiporter located on the apical membrane. (C) 1999 Elsevier Science Ltd. All rights reserved. | Notes: | Creighton Univ, Sch Med, Dept Biomed Sci, Omaha, NE 68178 USA. Limburgs Univ Ctr, B-3590 Diepenbeek, Belgium.Petzel, DH, Creighton Univ, Sch Med, Dept Biomed Sci, Omaha, NE 68178 USA. | Keywords: | Malpighian tubule; cyclicAMP; microfluorescence; pH; potassium | Document URI: | http://hdl.handle.net/1942/3088 | DOI: | 10.1016/S0022-1910(99)00076-1 | ISI #: | 000082195600002 | Type: | Journal Contribution | Validations: | ecoom 2000 |
Appears in Collections: | Research publications |
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