Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3096
Title: Cytokinin content and location in the leaves of the wild-type and transgenic tobacco plants
Authors: Veselov, SY
VALCKE, Roland 
Van Onckelen, H
Kudoyarova, GR
Issue Date: 1999
Publisher: MAIK NAUKA/INTERPERIODICA
Source: RUSSIAN JOURNAL OF PLANT PHYSIOLOGY, 46(1). p. 26-31
Abstract: Cytokinin content was assayed in the leaves of tobacco (Nicotiana tabacum L.) plants, nontransformed (wild type) and transformed with agenetic construct containing the light-inducible ipt gene, using liquid chromatography followed by mass spectrophotometry and by thin-layer chromatography combined with immunoenzyme assay. Immunocytological location of phytohormones was investigated after successively treating plant tissue sections with polyclonal antibodies against zeatin riboside, species-specific immunoglobulins labeled with colloid gold, and silver preparation. Cytokinin content in leaves of the transformed plants was higher than in those of wild-type plants. The bottom leaves of the former accumulated zeatin nucleotide. The contents of zeatin and zeatin riboside in the upper leaves exceeded that in the lower leaves both in transformed and wild-type plants. Staining agrees the contents of zeatin and zeatin riboside in the leaves were shown to correlate with the intensity of immunostaining of leaf sections. High zeatin content in the bottom leaves of transgenic plants did not affect the level of immunostaining.
Notes: Bashkortostan State Univ, Ufa 450074, Russia. Limburgs Univ Ctr, Diepenbeek, Belgium. Univ Antwerp, Wilrijk, Belgium. Russian Acad Sci, Ufa Res Ctr, Inst Biol, Ufa 450001, Russia.Veselov, SY, Bashkortostan State Univ, Ul Frunze 32, Ufa 450074, Russia.
Keywords: Nicotiana tabacum; cytokinins; immunocytochemistry; ipt gene; quantitative assay; transgenic plants
Document URI: http://hdl.handle.net/1942/3096
ISI #: 000079007700006
Type: Journal Contribution
Validations: ecoom 2000
Appears in Collections:Research publications

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