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http://hdl.handle.net/1942/31036| Title: | From lab-scale to lab-on-card: Loop-mediated isothermal amplification of biological samples | Authors: | ZAIN, Ashwaq LAKSSIR, Brahim RESSAMI, El Mostafa THOELEN, Ronald VREYS, Frederik JAFRANE, Lamiaa BENHASSOU, Hassan Ait |
Issue Date: | 2019 | Publisher: | Engineering Functional Interfaces | Source: | Engineering Functional Interfaces, Heverlee, Belgium, 8-9 july 2019 | Abstract: | This work aims to translate lab-scale techniques for the detection of diseases towards lab-on-card(LOC) devices. Indeed, these point-of-care(POC) tests allow detecting diseases in resource-limited areas. The necessary functions of the LOC can be split up into four different sections: extraction of the cells from the biological sample, lysis of the cells to harvest DNA, DNA amplification based on loop-mediated isothermal amplification(LAMP) and DNA detection by adding SYBR Green I nucleic acid stain. These functionalities have separately been tested on LOCs. The results of the individual functions show that a fully functional POC device is an achievable goal. | Keywords: | Lab-on-card(LOC);Point-of-care;single step loop-mediated isothermal amplification(SS-LAMP);Lysis;cell extraction;SYBR Green I | Document URI: | http://hdl.handle.net/1942/31036 | Category: | C2 | Type: | Conference Material |
| Appears in Collections: | Research publications |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Abstract_FrederikVreys.pdf | Conference material | 317.81 kB | Adobe PDF | View/Open |
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