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http://hdl.handle.net/1942/3132
Title: | Intracellular pH shifts in cultured kidney (A6) cells: effects on apical Na+ transport | Authors: | ZEISKE, Wolfgang SMETS, Ilse AMELOOT, Marcel STEELS, Paul VAN DRIESSCHE, Willy |
Issue Date: | 1999 | Publisher: | AMER PHYSIOLOGICAL SOC | Source: | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 277(3). p. C469-C479 | Abstract: | Intracellular pH shifts in cultured kidney (A6) cells: effects on apical Na+ transport. Am. J. Physiol. 277 (Cell Physiol. 46): C469-C479, 1999.-We report, for the epithelial Nat channel (ENaC) in A6 cells, the modulation by cell pH (pH(c)) of the transepithelial Na+ current (I-Na), the current through the individual Na+ channel (i), the open Nat channel density (N-o), and the kinetic parameters of the relationship between INa and the apical Naf concentration. The i and N-o, were evaluated from the Lorentzian IN, noise induced by the apical Na+ channel blocker 6-chloro-3,5-diaminopyrazine-2-carboxamide pH(c) shifts were induced, under strict and volume-controlled experimental conditions, by apical/basolateral NH4Cl pulses or basolateral arrest of the Na+/H+ exchanger (Nat removal; block by ethylisopropylamiloride) and were measured with the pH-sensitive probe 2',7'-bis(2-carboxyethyl)-5(6)-carboxy-fluorescein. The changes in pH, were positively correlated to changes in IN, and the apically dominated transepithelial conductance. The sole pH(c)-sensitive parameter underlying INa was N-o. Only the saturation value of the INa kinetics was subject to changes in pH(c). pH(c)-dependent changes in N-o may be caused by influencing P-o, the ENaC open probability, or/and the total channel number, N-T = N-o/P-o. | Notes: | Catholic Univ Louvain, Physiol Lab, B-3000 Louvain, Belgium. Limburgs Univ Ctr, Physiol Lab, B-3590 Diepenbeek, Belgium.Van Driessche, W, Catholic Univ Louvain, Physiol Lab, Campus Gasthuisberg, B-3000 Louvain, Belgium.Willy.VanDriessche@med.KULeuven.ac.be | Keywords: | noise analysis; single-channel current; epithelial sodium channel; ammonium; cell volume | Document URI: | http://hdl.handle.net/1942/3132 | ISI #: | 000082388800014 | Type: | Journal Contribution | Validations: | ecoom 2000 |
Appears in Collections: | Research publications |
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