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Title: | Comparison of Two Inoculation Methods of Endophytic Bacteria to Enhance Phytodegradation Efficacy of an Aged Petroleum Hydrocarbons Polluted Soil | Authors: | Pawlik, Malgorzata Plociniczak, Tomasz THIJS, Sofie Pintelon, Isabel VANGRONSVELD, Jaco Piotrowska-Seget, Zofia |
Issue Date: | 2020 | Publisher: | MDPI | Source: | AGRONOMY-BASEL, 10 (8) (Art N° 1196) | Abstract: | Endophyte-enhanced phytodegradation is a promising technology to clean up polluted soils. To improve the success rate of this nature-based remediation approach, it is important to advance the inoculation method as this has been shown to strongly affect the final outcome. However, studies evaluating inoculation strategies and their effect on hydrocarbon degradation are limited. This study aims to investigate two different manners of endophyte inoculation inLolium perennegrowing in an aged petroleum hydrocarbon polluted soil: (1) direct soil inoculation (SI), and (2) pre-inoculation of the caryopses followed by soil inoculation (PI). Different endophytic bacterial strains,Rhodococcus erythropolis5WK andRhizobiumsp. 10WK, were applied individually as well as in combination. Depending on the method of inoculation, the petroleum hydrocarbon (PHC) degradation potential was significantly different. The highest PHC removal was achieved after pre-inoculation of ryegrass caryopses with a consortium of both bacterial strains. Moreover, both strains established in the aged-polluted soil and could also colonize the roots and shoots ofL. perenne. Importantly, used endophytes showed the selective colonization of the environment compartments. Our findings show that the method of inoculation determines the efficiency of the phytodegradation process, especially the rate of PHC degradation. This study provides valuable information for choosing the most cost-effective and beneficial means to optimize phytodegradation. | Notes: | Pawlik, M (corresponding author), Univ Silesia Katowice, Fac Nat Sci, Inst Biol Biotechnol & Environm Protect, Jagiellonska 28, PL-40032 Katowice, Poland. malgorzata.pawlik@us.edu.pl; tomasz.plociniczak@us.edu.pl; sofie.thijs@uhasselt.be; isabel.pintelon@uantwerpen.be; jaco.vangronsveld@uhasselt.be; zofia.piotrowska-seget@us.edu.pl |
Other: | Pawlik, M (corresponding author), Univ Silesia Katowice, Fac Nat Sci, Inst Biol Biotechnol & Environm Protect, Jagiellonska 28, PL-40032 Katowice, Poland. malgorzata.pawlik@us.edu.pl; tomasz.plociniczak@us.edu.pl; sofie.thijs@uhasselt.be; isabel.pintelon@uantwerpen.be; jaco.vangronsveld@uhasselt.be; zofia.piotrowska-seget@us.edu.pl | Keywords: | endophytic bacteria;phytoremediation;petroleum hydrocarbon polluted soil;inoculation;pre-inoculation;green fluorescent protein;plant growth-promoting bacteria | Document URI: | http://hdl.handle.net/1942/32914 | ISSN: | 2073-4395 | e-ISSN: | 2073-4395 | DOI: | 10.3390/agronomy10081196 | ISI #: | WOS:000567295000001 | Rights: | 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2021 |
Appears in Collections: | Research publications |
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agronomy-10-01196.pdf | Published version | 2.45 MB | Adobe PDF | View/Open |
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