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Title: | An observational study of innate immune responses in patients with acute appendicitis | Authors: | PEETERS, Toon MARTENS, Sandrina D'ONOFRIO, Valentino STAPPERS, Mark VAN DER HILST, Jeroen HOUBEN, Bert ACHTEN, Ruth Joosten, Leo A. B. GYSSENS, Inge |
Issue Date: | 2020 | Publisher: | NATURE RESEARCH | Source: | Scientific Reports, 10 (1) (Art N° 17352) | Abstract: | Acute appendicitis is a common surgical emergency worldwide. Exaggerated immune responses could be associated with appendicitis. This study aimed at characterizing immune responses towards a large variety of gut commensals and pathogens, and pattern recognition receptor (PRR) ligands, and investigating the course of systemic inflammation in a prospective cohort of acute appendicitis patients. PBMC responses of 23 patients of the cohort and 23 healthy controls were characterized more than 8 months post-surgery. Serum cytokine levels were measured in 23 patients at the time of appendicitis and after one month. CRP, WBC and percentage of neutrophils were analyzed in the total cohort of 325 patients. No differences in PBMC responses were found between patients and controls. Stronger IL-10 responses were found following complicated appendicitis. A trend towards lower IL-8 responses was shown following gangrenous appendicitis. Serum IL-10 and IL-6 were significantly elevated at presentation, and IL-6, IL-8 and TNF-alpha levels were higher in complicated appendicitis. Routine biomarkers could predict severity of appendicitis with high specificities, but low sensitivities. Cytokine responses in patients following acute appendicitis did not differ from healthy controls. Higher serum cytokine levels were found in acute complicated and gangrenous cases. Further research into discriminative biomarkers is warranted. | Notes: | Gyssens, IC (corresponding author), Jessa Hosp, Dept Infect Dis & Immun, B-3500 Hasselt, Belgium.; Gyssens, IC (corresponding author), Hasselt Univ, Fac Med & Life Sci, B-3500 Hasselt, Belgium.; Gyssens, IC (corresponding author), Radboud Univ Nijmegen Med Ctr, Dept Internal Med, NL-6525 GA Nijmegen, Netherlands.; Gyssens, IC (corresponding author), Radboud Univ Nijmegen Med Ctr, Dept Internal Med AIG 463, POB 9101, NL-6500 HB Nijmegen, Netherlands. Inge.gyssens@radboudumc.nl |
Document URI: | http://hdl.handle.net/1942/32922 | ISSN: | 2045-2322 | e-ISSN: | 2045-2322 | DOI: | 10.1038/s41598-020-73798-3 | ISI #: | WOS:000582704600027 | Rights: | Open Access Tis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2021 |
Appears in Collections: | Research publications |
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s41598-020-73798-3.pdf | Published version | 1.99 MB | Adobe PDF | View/Open |
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