Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/32936
Title: | Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results | Authors: | Moller, Peter Azqueta, Amaya Boutet-Robinet, Elisa Koppen, Gudrun Bonassi, Stefano Milic, Mirta Gajski, Goran Costa, Solange Teixeira, Joao Paulo Costa Pereira, Cristiana Dusinska, Maria GODSCHALK, Roger Brunborg, Gunnar Gutzkow, Kristine B. Giovannelli, Lisa Cooke, Marcus S. Richling, Elke Laffon, Blanca Valdiglesias, Vanessa Basaran, Nursen Del Bo', Cristian Zegura, Bojana Novak, Matjaz Stopper, Helga Vodicka, Pavel Vodenkova, Sona de Andrade, Vanessa Moraes Sramkova, Monika Gabelova, Alena Collins, Andrew LANGIE, Sabine |
Issue Date: | 2020 | Publisher: | NATURE RESEARCH | Source: | Nature Protocols, 15 (12) , p. 3817 -3826 | Abstract: | The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers. Here, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA). | Notes: | Moller, P (corresponding author), Univ Copenhagen, Sect Environm Hlth, Dept Publ Hlth, Copenhagen, Denmark. pemo@sund.ku.dk |
Other: | Moller, P (corresponding author), Univ Copenhagen, Sect Environm Hlth, Dept Publ Hlth, Copenhagen, Denmark. pemo@sund.ku.dk | Document URI: | http://hdl.handle.net/1942/32936 | ISSN: | 1754-2189 | e-ISSN: | 1750-2799 | DOI: | 10.1038/s41596-020-0398-1 | ISI #: | WOS:000583953500001 | Rights: | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2021 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
s41596-020-0398-1.pdf | Published version | 935.35 kB | Adobe PDF | View/Open |
WEB OF SCIENCETM
Citations
215
checked on Oct 13, 2024
Page view(s)
30
checked on Sep 6, 2022
Download(s)
10
checked on Sep 6, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.