Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/33055
Title: PEGylating poly(p-phenylene vinylene)-based bioimaging nanoprobes
Authors: PETERS, Martijn 
DESTA, Derese 
SENECA, Senne 
REEKMANS, Gunter 
ADRIAENSENS, Peter 
NOBEN, Jean-Paul 
HELLINGS, Niels 
JUNKERS, Tanja 
ETHIRAJAN, Anitha 
Issue Date: 2021
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Source: JOURNAL OF COLLOID AND INTERFACE SCIENCE, 581 (B) , p. 566 -575
Abstract: Hypothesis: Conjugated polymer nanoparticles (CNPs) have attracted considerable attention within bioimaging due to their excellent optical properties and biocompatibility. However, unspecific adsorption of proteins hampers their effective use as advanced bioimaging probes. Controlled methodologies made possible tailor-made functional poly(p-phenylene vinylene), enabling one-pot synthesis of CNPs containing functional surface groups. Hence, it should be feasible to PEGylate these CNPs to tune the uptake by cell lines representative for the brain without imparting their optical properties. Experiments: CNPs consisting of the statistical copolymer 2-(50-methoxycarbonylpentyloxy)-5-methoxy-1,4-phenylenevinylene and poly(2-methoxy-5-(30,70-dimethoxyoctyloxy)-1,4-phenylenevinylene) were fabricated by miniemulsion solvent evaporation technique. Surface carboxylic acid groups were used to covalently attach amine-terminated polyethylene glycol (PEG) of different molecular weights. We investigated the effect of grafting CNPs with PEG chains on their intrinsic optical properties, protein adsorption behavior and uptake by representative brain cell lines. Findings: PEGylation did not affect the optical properties and biocompatibility of our CNPs. Moreover, a significant decrease in protein corona formation and unspecific uptake in central nervous system cell lines, depending on PEG chain length, was observed. This is the first report indicating that PEGylation does not affect the CNPs role as excellent bioimaging tools and can be adapted to tune biological interactions with brain cells. (C) 2020 Elsevier Inc. All rights reserved.
Notes: Ethirajan, A (corresponding author), Hasselt Univ, Inst Mat Res, Wetenschapspk 1, B-3590 Diepenbeek, Belgium.
anitha.ethirajan@uhasselt.be
Other: Ethirajan, A (corresponding author), Hasselt Univ, Inst Mat Res, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. anitha.ethirajan@uhasselt.be
Keywords: Conjugated polymers;Nanoparticles;CPM-PPV-co-MDMO-PPV;Protein corona;Optical properties;Bioimaging
Document URI: http://hdl.handle.net/1942/33055
ISSN: 0021-9797
e-ISSN: 1095-7103
DOI: 10.1016/j.jcis.2020.07.145
ISI #: WOS:000591642300012
Rights: 2020 Elsevier Inc. All rights reserved.
Category: A1
Type: Journal Contribution
Validations: ecoom 2021
Appears in Collections:Research publications

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