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http://hdl.handle.net/1942/3308
Title: | Potential misevaluation of the ground-state dissociation constant from fluorimetric titrations: Application to the ion indicators SBFI, PBFI, and Fura-2 | Authors: | Kowalczyk, A Boens, N Meuwis, K AMELOOT, Marcel |
Issue Date: | 1997 | Publisher: | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS | Source: | ANALYTICAL BIOCHEMISTRY, 245(1). p. 28-37 | Abstract: | A test based on time-resolved fluorescence experiments is proposed to assess the interference of an excited-state reaction with the fluorimetric determination of the ground-state dissociation constant K-d of ion subset of fluorescent indicator complexes. If an inflection point occurs in the plot of the fluorescence signal vs -log [ion] in the concentration range of the ion where both decay times are invariant, this inflection point can be associated With the correct K-d. In contrast, the inflection point(s) in the concentration range where the decay times vary cannot be attributed to K-d. The test is applied to the fluorescent ion indicators SBFI (for Na+), PBFI (for K+), and Fura-2 (for Ca2+). In all three cases the decay times are invariant in the physiological concentration ranges of the respective ions, indicating that the fluorimetrically determined K-d values are actually the true K-d values. (C) 1997 Academic Press. | Notes: | NICHOLAS COPERNICUS UNIV,INST PHYS,PL-87100 TORUN,POLAND. KATHOLIEKE UNIV LEUVEN,DEPT CHEM,B-3001 HEVERLEE,BELGIUM. LIMBURGS UNIV CTR,B-3590 DIEPENBEEK,BELGIUM. | Document URI: | http://hdl.handle.net/1942/3308 | DOI: | 10.1006/abio.1996.9932 | ISI #: | A1997WE92400004 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
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