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http://hdl.handle.net/1942/33177
Title: | Optimization of label-free nano LC-MS/MS analysis of the placental proteome | Authors: | LUYTEN, Leen Dieu, Marc Demazy, Catherine Fransolet, Maude NAWROT, Tim Renard, Patricia Debacq-Chainiaux, Florence |
Issue Date: | 2020 | Publisher: | W B SAUNDERS CO LTD | Source: | PLACENTA, 101 , p. 159 -162 | Abstract: | The placenta can be regarded as a mirror of the events to which the fetus is exposed during development. The placental proteome has been studied with several methodologies differing in sample handling, protein extraction, and processing. We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to the conditions studied. We recommend mechanical tissue disruption, blood removal prior to protein extraction, and FASP-based or in-gel digestion. | Notes: | Debacq-Chainiaux, F (corresponding author), Univ Namur UNamur, Unite Rech Biol Cellulaire URBC, Namur Res Inst Life Sci Narilis, Namur, Belgium. florence.chainiaux@unamur.be |
Other: | Debacq-Chainiaux, F (corresponding author), Univ Namur UNamur, Unite Rech Biol Cellulaire URBC, Namur Res Inst Life Sci Narilis, Namur, Belgium. florence.chainiaux@unamur.be | Keywords: | Placenta;Proteomics;Label-free nano LC-MS/MS | Document URI: | http://hdl.handle.net/1942/33177 | ISSN: | 0143-4004 | e-ISSN: | 1532-3102 | DOI: | 10.1016/j.placenta.2020.09.013 | ISI #: | WOS:000590600700021 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
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