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http://hdl.handle.net/1942/34183
Title: | Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9 | Authors: | Vitale, Maria Rosaria Zoeller, Johanna Eva Maria Jansch, Charline Janz, Anna Edenhofer, Frank Klopocki, Eva van den Hove, Daniel VANMIERLO, Tim Rivero, Olga Kasri, Nael Nadif Ziegler, Georg Christoph Lesch, Klaus-Peter |
Issue Date: | 2021 | Publisher: | ELSEVIER | Source: | Stem Cell Research, 51 (Art N° 102169) | Abstract: | Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13(+/-)) and a CDH13 null mutant (CDH13(-/-)) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype. | Notes: | Vitale, MR; Lesch, KP (corresponding author), Univ Hosp Wurzburg, Ctr Mental Hlth, Div Mol Psychiat, Margarete Hoppel Pl 1, D-97080 Wurzburg, Germany. Vitale_M@ukw.de; kplesch@mail.uni-wuerzburg.de |
Other: | Vitale, MR; Lesch, KP (corresponding author), Univ Hosp Wurzburg, Ctr Mental Hlth, Div Mol Psychiat, Margarete Hoppel Pl 1, D-97080 Wurzburg, Germany. Vitale_M@ukw.de; kplesch@mail.uni-wuerzburg.de | Keywords: | CRISPR-Cas Systems;Cadherins;Female;Heterozygote;Humans;Middle Aged;Induced Pluripotent Stem Cells;Neurodevelopmental Disorders | Document URI: | http://hdl.handle.net/1942/34183 | ISSN: | 1873-5061 | e-ISSN: | 1876-7753 | DOI: | 10.1016/j.scr.2021.102169 | ISI #: | WOS:000629039100019 | Rights: | 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
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1-s20-S1873506121000155-main.pdf | Published version | 2.29 MB | Adobe PDF | View/Open |
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