Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/34796
Title: Label-free detection of uptake, accumulation, and translocation of diesel exhaust particles in ex vivo perfused human placenta
Authors: BONGAERTS, Eva 
Aengenheister, L
Dugershaw, BB
Manser, P
Roeffaers, MBJ
AMELOOT, Marcel 
NAWROT, Tim 
BOVE, Hannelore 
Buerki-Thurnherr, T
Issue Date: 2021
Publisher: BMC
Source: Journal of nanobiotechnology, 19 (1) , (Art N° 144)
Abstract: BackgroundPregnant women and developing fetuses comprise a particularly vulnerable population as multiple studies have shown associations between prenatal air pollution exposure and adverse pregnancy outcomes. However, the mechanisms underlying the observed developmental toxicity are mostly unknown, in particular, if pollution particles can cross the human placenta to reach the fetal circulation.ResultsHere, we investigated the accumulation and translocation of diesel exhaust particles (DEPs), as a model particle for combustion-derived pollution, in human perfused placentae using label-free detection by femtosecond pulsed laser illumination. The results do not reveal a significant particle transfer across term placentae within 6 h of perfusion. However, DEPs accumulate in placental tissue, especially in the syncytiotrophoblast layer that mediates a wealth of essential functions to support and maintain a successful pregnancy. Furthermore, DEPs are found in placental macrophages and fetal endothelial cells, showing that some particles can overcome the syncytiotrophoblasts to reach the fetal capillaries. Few particles are also observed inside fetal microvessels.ConclusionsOverall, we show that DEPs accumulate in key cell types of the placental tissue and can cross the human placenta, although in limited amounts. These findings are crucial for risk assessment and protection of pregnant women and highlight the urgent need for further research on the direct and indirect placenta-mediated developmental toxicity of ambient particulates.
Other: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Keywords: Environmental pollution;Diesel exhaust particles;In utero exposure;Ex vivo placental perfusion;Nanosafety
Document URI: http://hdl.handle.net/1942/34796
e-ISSN: 1477-3155
DOI: 10.1186/s12951-021-00886-5
ISI #: 000655044700006
Rights: The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativeco mmons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/ zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data
Category: A1
Type: Journal Contribution
Validations: ecoom 2022
Appears in Collections:Research publications

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