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http://hdl.handle.net/1942/35778
Title: | Rectal organoid morphology analysis (ROMA) as a promising diagnostic tool in cystic fibrosis | Authors: | Cuyx, Senne Santo Ramalho, Anabela Corthout, Nikky FIEUWS, Steffen Furstova, Eva Arnauts, Kaline Ferrante, Marc Verfaillie, Catherine Munck, Sebastian Boon, Mieke Proesmans, Marijke Dupont, Lieven De Boeck, Kris Vermeulen, Francois |
Issue Date: | 2021 | Publisher: | BMJ PUBLISHING GROUP | Source: | THORAX, 76(11), p. 1146-1149. | Abstract: | Diagnosing cystic fibrosis (CF) when sweat chloride is not in the CF range and less than 2 disease-causing CFTR mutations are found requires physiological CFTR assays, which are not always feasible or available. We developed a new physiological CFTR assay based on the morphological differences between rectal organoids from subjects with and without CF. In organoids from 167 subjects with and 22 without CF, two parameters derived from a semi-automated image analysis protocol (rectal organoid morphology analysis, ROMA) fully discriminated CF subjects with two disease-causing mutations from non-CF subjects (p<0.001). ROMA, feasible at all ages, can be centralised to improve standardisation. | Notes: | Vermeulen, F (corresponding author), Katholieke Univ Leuven, Woman & Child Unit, CF Res Lab, Dept Dev & Regenerat, Leuven, Flanders, Belgium. francois.vermeulen@uzleuven.be |
Keywords: | cystic fibrosis | Document URI: | http://hdl.handle.net/1942/35778 | ISSN: | 0040-6376 | e-ISSN: | 1468-3296 | DOI: | 10.1136/thoraxjnl-2020-216368 | ISI #: | WOS:000709895600014 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
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