Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/35980
Title: Carnosine, oxidative and carbonyl stress, antioxidants, and muscle fiber characteristics of quadriceps muscle of patients with COPD
Authors: DE BRANDT, Jana 
BURTIN, Chris 
Pomies, P.
VANDENABEELE, Frank 
VERBOVEN, Kenneth 
AUMANN, Joseph 
Blancquaert, L.
Everaert, I
VAN RYCKEGHEM, Lisa 
COPS, Jirka 
Hayot, M.
SPRUIT, Martijn A. 
Derave, W.
Issue Date: 2021
Publisher: AMER PHYSIOLOGICAL SOC
Source: Journal of applied physiology (1985), 131 (4) , p. 1230 -1240
Abstract: Oxidative/carbonyl stress is elevated in lower-limb muscles of patients with chronic obstructive pulmonary disease (COPD). Carnosine is a skeletal muscle antioxidant particularly present in fast-twitch fibers. The aims of the present study were to compare muscle carnosine, oxidative/carbonyl stress, antioxidants, and fiber characteristics between patients with COPD and healthy controls (HCs) and between patients after stratification for airflow limitation (mild/moderate vs. severe/very severe), as well as to investigate correlates of carnosine in patients with COPD. A vastus lateralis muscle biopsy was obtained from 40 patients with stable COPD and 20 age- and sex-matched HCs. Carnosine, oxidative/carbonyl stress, antioxidants, fiber characteristics, quadriceps strength and endurance (QE), V(O2)peak (incremental cycle test), and physical activity (PA) were determined. Patients with COPD had a similar carnosine concentration [4.16mmol/kg wet weight (WW; SD = 1.93)] to HCs [4.64mmol/kg WW (SD = 1.71)] and significantly higher percentage of fast-twitch fibers and lower QE, V(O2)peak, and PA versus HCs. Patients with severe/very severe COPD had a 31% lower carnosine concentration [3.24mmol/kg WW (SD = 1.79); n = 15] versus patients with mild/moderate COPD [4.71mmol/kg WW (SD = 1.83); n = 25; P = 0.02] and significantly lower V(O2)peak and PA versus patients with mild/moderate COPD. Carnosine correlated significantly with QE (r(s) = 0.427), V(O2)peak (r(s) = 0.334), PA (r(s) = 0.379), and lung function parameters in patients with COPD. In conclusion, despite having the highest proportion of fast-twitch fibers, patients with severe/very severe COPD displayed a 31% lower muscle carnosine concentration compared with patients with mild/moderate COPD. As no other markers of oxidative/carbonyl stress or antioxidants were affected, the observed carnosine deficiency is thought to be a possible first sign of muscle redox balance abnormalities. NEW & NOTEWORTHY Carnosine, particularly present in fast-twitch fibers, was investigated in the quadriceps of patients with chronic obstructive pulmonary disease (COPD). Carnosine concentration was similar between patients with COPD and healthy controls but was 31% lower in patients with severe/very severe COPD, despite their high proportion of fast-twitch fibers, versus patients with mild/moderate COPD. As no other markers of oxidative/carbonyl stress or antioxidants were affected, the observed carnosine deficiency is thought to be a possible first sign of muscle redox balance abnormalities.
Notes: Burtin, C (corresponding author), Hasselt Univ, Fac Rehabil Sci, REVAL Rehabil Res Ctr, Diepenbeek, Belgium.; Burtin, C (corresponding author), Hasselt Univ, Fac Med & Life Sci, BIOMED Biomed Res Inst, Diepenbeek, Belgium.
chris.burtin@uhasselt.be
Keywords: carbonyl stress;carnosine;chronic obstructive pulmonary disease;oxidative stress;quadriceps
Document URI: http://hdl.handle.net/1942/35980
ISSN: 8750-7587
e-ISSN: 1522-1601
DOI: 10.1152/japplphysiol.00200.2021
ISI #: WOS:000702916400001
Rights: 2021 the American Physiological Society.
Category: A1
Type: Journal Contribution
Validations: ecoom 2022
Appears in Collections:Research publications

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