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Title: | Carnosine, oxidative and carbonyl stress, antioxidants, and muscle fiber characteristics of quadriceps muscle of patients with COPD | Authors: | DE BRANDT, Jana BURTIN, Chris Pomies, P. VANDENABEELE, Frank VERBOVEN, Kenneth AUMANN, Joseph Blancquaert, L. Everaert, I VAN RYCKEGHEM, Lisa COPS, Jirka Hayot, M. SPRUIT, Martijn A. Derave, W. |
Issue Date: | 2021 | Publisher: | AMER PHYSIOLOGICAL SOC | Source: | Journal of applied physiology (1985), 131 (4) , p. 1230 -1240 | Abstract: | Oxidative/carbonyl stress is elevated in lower-limb muscles of patients with chronic obstructive pulmonary disease (COPD). Carnosine is a skeletal muscle antioxidant particularly present in fast-twitch fibers. The aims of the present study were to compare muscle carnosine, oxidative/carbonyl stress, antioxidants, and fiber characteristics between patients with COPD and healthy controls (HCs) and between patients after stratification for airflow limitation (mild/moderate vs. severe/very severe), as well as to investigate correlates of carnosine in patients with COPD. A vastus lateralis muscle biopsy was obtained from 40 patients with stable COPD and 20 age- and sex-matched HCs. Carnosine, oxidative/carbonyl stress, antioxidants, fiber characteristics, quadriceps strength and endurance (QE), V(O2)peak (incremental cycle test), and physical activity (PA) were determined. Patients with COPD had a similar carnosine concentration [4.16mmol/kg wet weight (WW; SD = 1.93)] to HCs [4.64mmol/kg WW (SD = 1.71)] and significantly higher percentage of fast-twitch fibers and lower QE, V(O2)peak, and PA versus HCs. Patients with severe/very severe COPD had a 31% lower carnosine concentration [3.24mmol/kg WW (SD = 1.79); n = 15] versus patients with mild/moderate COPD [4.71mmol/kg WW (SD = 1.83); n = 25; P = 0.02] and significantly lower V(O2)peak and PA versus patients with mild/moderate COPD. Carnosine correlated significantly with QE (r(s) = 0.427), V(O2)peak (r(s) = 0.334), PA (r(s) = 0.379), and lung function parameters in patients with COPD. In conclusion, despite having the highest proportion of fast-twitch fibers, patients with severe/very severe COPD displayed a 31% lower muscle carnosine concentration compared with patients with mild/moderate COPD. As no other markers of oxidative/carbonyl stress or antioxidants were affected, the observed carnosine deficiency is thought to be a possible first sign of muscle redox balance abnormalities. NEW & NOTEWORTHY Carnosine, particularly present in fast-twitch fibers, was investigated in the quadriceps of patients with chronic obstructive pulmonary disease (COPD). Carnosine concentration was similar between patients with COPD and healthy controls but was 31% lower in patients with severe/very severe COPD, despite their high proportion of fast-twitch fibers, versus patients with mild/moderate COPD. As no other markers of oxidative/carbonyl stress or antioxidants were affected, the observed carnosine deficiency is thought to be a possible first sign of muscle redox balance abnormalities. | Notes: | Burtin, C (corresponding author), Hasselt Univ, Fac Rehabil Sci, REVAL Rehabil Res Ctr, Diepenbeek, Belgium.; Burtin, C (corresponding author), Hasselt Univ, Fac Med & Life Sci, BIOMED Biomed Res Inst, Diepenbeek, Belgium. chris.burtin@uhasselt.be |
Keywords: | carbonyl stress;carnosine;chronic obstructive pulmonary disease;oxidative stress;quadriceps | Document URI: | http://hdl.handle.net/1942/35980 | ISSN: | 8750-7587 | e-ISSN: | 1522-1601 | DOI: | 10.1152/japplphysiol.00200.2021 | ISI #: | WOS:000702916400001 | Rights: | 2021 the American Physiological Society. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
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japplphysiol.00200.2021.pdf | Peer-reviewed author version | 9.98 MB | Adobe PDF | View/Open |
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