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Title: | Metabolic reprogramming of Pseudomonas aeruginosa by phage-based quorum sensing modulation | Authors: | Hendrix , Hanne Zimmermann-Kogadeeva, Maria Zimmermann, Michael Sauer, Uwe De Smet , Jeroen Muchez, Laurens Lissens, Maries Staes, Ines Voet, Marleen Wagemans , Jeroen Ceyssens, Pieter-Jan NOBEN, Jean-Paul Aertsen, Abram Lavigne, Rob |
Issue Date: | 2022 | Publisher: | CELL PRESS | Source: | Cell Reports, 38 (7) (Art N° 110372) | Abstract: | The Pseudomonas quinolone signal (PQS) is a multifunctional quorum sensing molecule of key importance to P. aeruginosa. Here, we report that the lytic Pseudomonas bacterial virus LUZ19 targets this population density-dependent signaling system by expressing quorum sensing targeting protein (Qst) early during infection. We demonstrate that Qst interacts with PqsD, a key host quinolone signal biosynthesis pathway enzyme, resulting in decreased levels of PQS and its precursor 2-heptyl-4(1H)-quinolone. The lack of a functional PqsD enzyme impairs LUZ19 infection but is restored by external supplementation of 2-heptyl-4(1H)-quinolone, suggesting that LUZ19 exploits the PQS system for successful infection. We establish a broad functional interaction network of Qst, which includes enzymes of cofactor biosynthesis pathways (CoaC/ThiD) and a non-ribosomal peptide synthetase pathway (PA1217). Qst therefore represents an exquisite example of intricate reprogramming of the bacterium by a phage, which may be further exploited as tool to combat antibiotic resistant bacterial pathogens. | Notes: | Lavigne, R (corresponding author), Katholieke Univ Leuven, Dept Biosyst, Lab Gene Technol, B-3001 Heverlee, Belgium. rob.lavigne@kuleuven.be |
Document URI: | http://hdl.handle.net/1942/36952 | ISSN: | 2211-1247 | e-ISSN: | 2211-1247 | DOI: | 10.1016/j.celrep.2022.110372 | ISI #: | WOS:000759253300004 | Rights: | 2022 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2023 |
Appears in Collections: | Research publications |
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Metabolic reprogramming of Pseudomonas aeruginosa by phage-based quorum sensing modulation.pdf | Published version | 3.04 MB | Adobe PDF | View/Open |
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