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Title: | Experimental early‐life febrile seizures cause a sustained increase in excitatory neurotransmission in newborn dentate granule cells | Authors: | HOOGLAND, Govert RAIJMAKERS, Marjolein CLYNEN, Elke BRONE, Bert RIGO, Jean-Michel SWIJSEN, Ann |
Issue Date: | 2022 | Publisher: | WILEY | Source: | Brain and Behavior, (Art N° e2505) | Status: | Early view | Abstract: | Prolonged febrile seizures (FS) are a risk factor for the development of hippocampal-associated temporal lobe epilepsy. The dentate gyrus is the major gateway to the hippocampal network and one of the sites in the brain where neurogenesis continues postnatally. Previously, we found that experimental FS increase the survival rate and structural integration of newborn dentate granule cells (DGCs). In addition, mature post-FS born DGCs express an altered receptor panel. Here, we aimed to study if these molecular and structural changes are accompanied by an altered cellular functioning. Experimental FS were induced by hyperthermia in 10-days-old Sprague-Dawley rats. Proliferating progenitor cells were labeled the next day by injecting green fluorescent protein expressing retroviral particles bilaterally in the dentate gyri. Eight weeks later, spontaneous excitatory and inhibitory postsynaptic events (sEPSCs and sIPSCs, respectively) were recorded from labeled DGCs using the whole-cell patch-clamp technique. Experimental FS resulted in a robust decrease of the inter event interval (p < .0001) and a small decrease of the amplitude of sEPSCs (p < .001). Collectively the spontaneous excitatory charge transfer increased (p < .01). Experimental FS also slightly increased the frequency of sIPSCs (p < .05), while the amplitude of these events decreased strongly (p < .0001). The net inhibitory charge transfer remained unchanged. Experimental, early-life FS have a long-term effect on post-FS born DGCs, as they display an increased spontaneous excitatory input when matured. It remains to be established if this presents a mechanism for FS-induced epileptogenesis. | Notes: | Rigo, JM (corresponding author), Martelarenlaan 42, B-3500 Hasselt, Belgium. jeanmichel.rigo@uhasselt.be |
Keywords: | dentate granule cells;epileptogenesis;febrile seizures;hyperexcitability;neurogenesis | Document URI: | http://hdl.handle.net/1942/36992 | ISSN: | 2162-3279 | e-ISSN: | 2162-3279 | DOI: | 10.1002/brb3.2505 | ISI #: | WOS:000758686800001 | Rights: | 2022 The Authors. Brain and Behavior published by Wiley Periodicals LLC This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2023 |
Appears in Collections: | Research publications |
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