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Title: | New nanostructures inhibiting human mannose binding lectin identified by a novel surface plasmon resonance assay | Authors: | Erol, Gizem Schmidt, Patricia Perez PANCARO, Alessia Diaz, Javier M. Melo Barrientos, Africa G. Porter, John Polito, Laura Szymonik, Michal Nelissen, Inge Spencer, Daniel I. R. Piotti, Arianna Beeg, Marten De Simoni, Maria-Grazia Fumagalli, Stefano Gobbi, Marco |
Issue Date: | 2022 | Publisher: | ELSEVIER SCIENCE SA | Source: | Sensors and actuators. B, Chemical (Print), 360 (Art N° 131661) | Abstract: | Circulating mannose-binding lectin (MBL) plays an important role in the progression of tissue damage caused by ischemic events, an account of its high-affinity, multivalent binding to carbohydrate arrays exposed on damaged endothelium (damage-associated molecular patterns, DAMPS). MBL inhibitors have therefore been proposed as a novel protective therapeutic strategy to prevent secondary injury. We developed a new, convenient, robust surface plasmon resonance (SPR) assay for in vitro screening of compounds interfering with the binding of native human MBL to an appropriate chip surface functionalized with a pattern of sugar moieties mimicking DAMPS. We also characterized the procedure to regenerate the chip surface after each experimental session, accomplished by sequential cleaning with piranha solution followed by UV exposure. The SPR assay detects the specific binding of human recombinant MBL and native MBL present in human serum, and can identify inhibitors of this binding. We observed inhibitory effects of mannose (IC50 5 mM), of a nine mannose residues carrying glycan (IC50 0.33 mg/mL, corresponding to ~ 175 mu M), and mainly mannose-coated gold nanoparticles (IC50 1.1 mu g /mL). These in vitro results serve as a basis for testing the protective properties of these molecules/nanoparticles later inthe more expensive and time-consuming studies in cells and animal models of MBL mediated-injuries. | Notes: | Gobbi, M (corresponding author), Ist Ric Farmacol Mario Negri IRCCS, Via Mario Negri 2, I-20156 Milan, Italy. marco.gobbi@marionegri.it |
Keywords: | Mannose binding lectin;Glycans;Nanoparticles;Surface plasmon resonance | Document URI: | http://hdl.handle.net/1942/37667 | e-ISSN: | 0925-4005 | DOI: | 10.1016/j.snb.2022.131661 | ISI #: | 000812315500006 | Rights: | 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). The authors wish to thank Mrs. Judith Baggott for English stylistic editing. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2023 |
Appears in Collections: | Research publications |
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