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http://hdl.handle.net/1942/39715| Title: | Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing | Authors: | DIOUF, David Vitale, Maria Rosaria Zoeller, Johanna Eva Maria Pineau, Ana -Magdalena Klopocki, Eva Hamann, Catharina Ziegler, Georg Christoph VANMIERLO, Tim Van den Hove, Daniel Lesch, Klaus-Peter |
Issue Date: | 2023 | Publisher: | ELSEVIER | Source: | Stem Cell Research, 67 (Art N° 103038) | Abstract: | Fibroblasts isolated from a skin biopsy of a healthy individual were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate an isogenic cell line carrying an inactivation of ST3GAL3, a risk gene associated with neurodevelopmental and psychiatric disorders. This ST3GAL3 null mutant (ST3GAL3-/-) iPSC line, which displays the expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal karyotype, is a powerful tool to investigate the impact of deficient sialylation of glycoproteins in neural development and plasticity. | Notes: | Diouf, D; Lesch, KP (corresponding author), Univ Hosp Wurzburg, Ctr Mental Hlth, Div Mol Psychiat, Margarete Hoppel Pl 1, D-97080 Wurzburg, Germany. Diouf_D@ukw.de; kplesch@mail.uni-wuerzburg.de |
Keywords: | Humans;Gene Editing;CRISPR-Cas Systems;Cell Differentiation;Cell Line;Induced Pluripotent Stem Cells | Document URI: | http://hdl.handle.net/1942/39715 | ISSN: | 1873-5061 | e-ISSN: | 1876-7753 | DOI: | 10.1016/j.scr.2023.103038 | ISI #: | 000930528500001 | Rights: | 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | Category: | A1 | Type: | Journal Contribution |
| Appears in Collections: | Research publications |
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| Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR_Cas9 genome editing.pdf | Published version | 3.5 MB | Adobe PDF | View/Open |
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