Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/40106
Title: Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development
Authors: HERMANS, Florian 
HEMERYCK, Lara 
Bueds, Celine
Torres Pereiro, Marc
HASEVOETS, Steffie 
Kobayashi, Hiroto
Lambrechts, Diether
LAMBRICHTS, Ivo 
BRONCKAERS, Annelies 
Vankelecom, Hugo
Issue Date: 2023
Publisher: Cell Press
Source: Stem Cell Reports, 18 (5) , p. 1166 -1181
Abstract: Organoid models provide powerful tools to study tissue biology and development in a dish. Presently, organoids have not yet been developed from mouse tooth. Here, we established tooth organoids (TOs) from early-postnatal mouse molar and incisor, which are long-term expandable, express dental epithelium stem cell (DESC) markers, and recapitulate key properties of the dental epithelium in a tooth-type-specific manner. TOs display in vitro differentiation capacity toward ameloblast-resembling cells, even more pronounced in assembloids in which dental mesenchymal (pulp) stem cells are combined with the organoid DESCs. Single-cell transcriptomics supports this developmental potential and reveals co-differentiation into junctional epithelium- and odontoblast-/cementoblast-like cells in the assembloids. Finally, TOs survive and show ameloblast-resembling differentiation also in vivo. The developed organoid models provide new tools to study mouse tooth-type-specific biology and development and gain deeper molecular and functional insights that may eventually help to achieve future human biological tooth repair and replacement.
Keywords: ameloblasts;assembloids;dental epithelium;incisors;molars;organoids;single-cell RNA-sequencing;stem cells;tooth development;Animals;Mice;Humans;Molar;Cell Differentiation;Organoids;Biology;Incisor;Ameloblasts
Document URI: http://hdl.handle.net/1942/40106
Link to publication/dataset: https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(23)00099-1
ISSN: 2213-6711
e-ISSN: 2213-6711
DOI: 10.1016/j.stemcr.2023.03.011
ISI #: 001017408400001
Datasets of the publication: 10.17632/2kskdknngb.1
Datasets of the publication: https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-12557
https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-12544
Rights: 2023 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Category: A1
Type: Journal Contribution
Validations: ecoom 2024
Appears in Collections:Research publications

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