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Title: | Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development | Authors: | HERMANS, Florian HEMERYCK, Lara Bueds, Celine Torres Pereiro, Marc HASEVOETS, Steffie Kobayashi, Hiroto Lambrechts, Diether LAMBRICHTS, Ivo BRONCKAERS, Annelies Vankelecom, Hugo |
Issue Date: | 2023 | Publisher: | Cell Press | Source: | Stem Cell Reports, 18 (5) , p. 1166 -1181 | Abstract: | Organoid models provide powerful tools to study tissue biology and development in a dish. Presently, organoids have not yet been developed from mouse tooth. Here, we established tooth organoids (TOs) from early-postnatal mouse molar and incisor, which are long-term expandable, express dental epithelium stem cell (DESC) markers, and recapitulate key properties of the dental epithelium in a tooth-type-specific manner. TOs display in vitro differentiation capacity toward ameloblast-resembling cells, even more pronounced in assembloids in which dental mesenchymal (pulp) stem cells are combined with the organoid DESCs. Single-cell transcriptomics supports this developmental potential and reveals co-differentiation into junctional epithelium- and odontoblast-/cementoblast-like cells in the assembloids. Finally, TOs survive and show ameloblast-resembling differentiation also in vivo. The developed organoid models provide new tools to study mouse tooth-type-specific biology and development and gain deeper molecular and functional insights that may eventually help to achieve future human biological tooth repair and replacement. | Keywords: | ameloblasts;assembloids;dental epithelium;incisors;molars;organoids;single-cell RNA-sequencing;stem cells;tooth development;Animals;Mice;Humans;Molar;Cell Differentiation;Organoids;Biology;Incisor;Ameloblasts | Document URI: | http://hdl.handle.net/1942/40106 | Link to publication/dataset: | https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(23)00099-1 | ISSN: | 2213-6711 | e-ISSN: | 2213-6711 | DOI: | 10.1016/j.stemcr.2023.03.011 | ISI #: | 001017408400001 | Datasets of the publication: | 10.17632/2kskdknngb.1 | Datasets of the publication: | https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-12557 https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-12544 |
Rights: | 2023 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2024 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Hermans Stem Cell Reports 2023 - main.pdf | Published version | 5.83 MB | Adobe PDF | View/Open |
Hermans Stem Cell Reports 2023 - supplemental.pdf | Supplementary material | 7.51 MB | Adobe PDF | View/Open |
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