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http://hdl.handle.net/1942/40383
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DC Field | Value | Language |
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dc.contributor.author | Li, ZL | - |
dc.contributor.author | BOESMANS, Werend | - |
dc.contributor.author | Kazwiny, Y | - |
dc.contributor.author | Hao, MM | - |
dc.contributor.author | Vanden Berghe, P | - |
dc.date.accessioned | 2023-06-13T15:33:55Z | - |
dc.date.available | 2023-06-13T15:33:55Z | - |
dc.date.issued | 2022 | - |
dc.date.submitted | 2023-06-06T10:14:18Z | - |
dc.identifier.citation | AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 323 (4) , p. G341 -G347 | - |
dc.identifier.issn | 0193-1857 | - |
dc.identifier.uri | http://hdl.handle.net/1942/40383 | - |
dc.description.abstract | Live calcium imaging is often used as a proxy for electrophysiological measurements and has been a valuable tool that allows simul-taneous analysis of neuronal activity in multiple cells at the population level. In the enteric nervous system, there are two main elec-trophysiological classes of neurons, after-hyperpolarizing (AH)-and synaptic (S)-neurons, which have been shown to have different calcium handling mechanisms. However, they are rarely considered separately in calcium imaging experiments. A handful of studies have shown that in guinea pig, a calcium transient will accompany a single action potential in AH-neurons, but multiple action poten-tials are required to generate a calcium transient in S-neurons. How this translates to different modes of cellular depolarization and whether this is consistent across species is unknown. In this study, we used simultaneous whole-cell patch-clamp electrophysiology together with calcium imaging to investigate how enteric neurons respond to different modes of depolarization. Using both traditional (4 Hz) and also high-speed (1,000 Hz) imaging techniques, we found that single action potentials elicit calcium transients in both AH-neurons and S-neurons. Subthreshold membrane depolarizations were also able to elicit calcium transients, although calcium responses were generally amplified if an action potential was present. Furthermore, we identified that responses to nicotinic acetyl-choline receptor stimulation can be used to distinguish between AH-and S-neurons in calcium imaging. NEW & NOTEWORTHY Live calcium imaging is an important tool for investigating enteric nervous system (ENS) function. Previous studies have shown that multiple action potentials are needed to generate a calcium response in S-neurons, which has important implications for the interpretation of calcium imaging data. Here, we show that in mouse myenteric neurons, calcium transients are elicited by single action potentials in both AH-and S-neurons. In addition, nicotinic acetylcholine receptor stimula-tion can be used to distinguish between these two classes. | - |
dc.language.iso | en | - |
dc.publisher | AMER PHYSIOLOGICAL SOC | - |
dc.subject.other | calcium imaging | - |
dc.subject.other | electrophysiology | - |
dc.subject.other | enteric nervous system | - |
dc.subject.other | neuronal activity | - |
dc.subject.other | patch -clamp | - |
dc.title | Simultaneous whole-cell patch-clamp and calcium imaging on myenteric neurons | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | G347 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | G341 | - |
dc.identifier.volume | 323 | - |
local.bibliographicCitation.jcat | A1 | - |
local.publisher.place | 6120 Executive Blvd, Suite 600, Rockville, MD, UNITED STATES | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1152/ajpgi.00162.2022 | - |
dc.identifier.isi | 000885977300005 | - |
dc.identifier.eissn | 1522-1547 | - |
dc.identifier.eissn | 1522-1547 | - |
local.provider.type | Web of Science | - |
local.uhasselt.international | yes | - |
item.fullcitation | Li, ZL; BOESMANS, Werend; Kazwiny, Y; Hao, MM & Vanden Berghe, P (2022) Simultaneous whole-cell patch-clamp and calcium imaging on myenteric neurons. In: AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 323 (4) , p. G341 -G347. | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2023 | - |
item.contributor | Li, ZL | - |
item.contributor | BOESMANS, Werend | - |
item.contributor | Kazwiny, Y | - |
item.contributor | Hao, MM | - |
item.contributor | Vanden Berghe, P | - |
item.accessRights | Open Access | - |
crisitem.journal.issn | 0193-1857 | - |
crisitem.journal.eissn | 1522-1547 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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ajpgi.00162.2022.pdf Restricted Access | Published version | 1.38 MB | Adobe PDF | View/Open Request a copy |
Li et al AJPGI 2022.pdf | Peer-reviewed author version | 1.65 MB | Adobe PDF | View/Open |
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