Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/40383
Title: | Simultaneous whole-cell patch-clamp and calcium imaging on myenteric neurons | Authors: | Li, ZL BOESMANS, Werend Kazwiny, Y Hao, MM Vanden Berghe, P |
Issue Date: | 2022 | Publisher: | AMER PHYSIOLOGICAL SOC | Source: | AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 323 (4) , p. G341 -G347 | Abstract: | Live calcium imaging is often used as a proxy for electrophysiological measurements and has been a valuable tool that allows simul-taneous analysis of neuronal activity in multiple cells at the population level. In the enteric nervous system, there are two main elec-trophysiological classes of neurons, after-hyperpolarizing (AH)-and synaptic (S)-neurons, which have been shown to have different calcium handling mechanisms. However, they are rarely considered separately in calcium imaging experiments. A handful of studies have shown that in guinea pig, a calcium transient will accompany a single action potential in AH-neurons, but multiple action poten-tials are required to generate a calcium transient in S-neurons. How this translates to different modes of cellular depolarization and whether this is consistent across species is unknown. In this study, we used simultaneous whole-cell patch-clamp electrophysiology together with calcium imaging to investigate how enteric neurons respond to different modes of depolarization. Using both traditional (4 Hz) and also high-speed (1,000 Hz) imaging techniques, we found that single action potentials elicit calcium transients in both AH-neurons and S-neurons. Subthreshold membrane depolarizations were also able to elicit calcium transients, although calcium responses were generally amplified if an action potential was present. Furthermore, we identified that responses to nicotinic acetyl-choline receptor stimulation can be used to distinguish between AH-and S-neurons in calcium imaging. NEW & NOTEWORTHY Live calcium imaging is an important tool for investigating enteric nervous system (ENS) function. Previous studies have shown that multiple action potentials are needed to generate a calcium response in S-neurons, which has important implications for the interpretation of calcium imaging data. Here, we show that in mouse myenteric neurons, calcium transients are elicited by single action potentials in both AH-and S-neurons. In addition, nicotinic acetylcholine receptor stimula-tion can be used to distinguish between these two classes. | Keywords: | calcium imaging;electrophysiology;enteric nervous system;neuronal activity;patch -clamp | Document URI: | http://hdl.handle.net/1942/40383 | ISSN: | 0193-1857 | e-ISSN: | 1522-1547 | DOI: | 10.1152/ajpgi.00162.2022 | ISI #: | 000885977300005 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2023 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
ajpgi.00162.2022.pdf Restricted Access | Published version | 1.38 MB | Adobe PDF | View/Open Request a copy |
Li et al AJPGI 2022.pdf | Peer-reviewed author version | 1.65 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.