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Title: | Stability of supported hybrid lipid bilayers on chemically and topographically-modified surfaces | Authors: | Bar, Laure Villanueva, Martin Eduardo Martin, Claudio VALENCIA RAMIREZ, Andrea Goole, Jonathan RENNER, Frank LOSADA-PEREZ, Patricia |
Issue Date: | 2023 | Publisher: | ELSEVIER | Source: | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 664 (Art N° 131125) | Abstract: | Hybrid lipid bilayers are a particular case of supported lipid bilayers with the two monolayer leaflets composed by different types of molecules. These nanostructures can be produced in a well-controlled array fashion and are suitable for the study of biomembrane-related phenomena via electrochemical or plasmonic sensing. Understanding how the underlying solid surface affects the supported membrane formation and organization is necessary for the potential use of these hybrid platforms in applications for which surfaces are not flat and topographically complex. Here we assess the role of lipid phase, substrate surface energy and topography on the formation and stability of hybrid supported membranes from vesicle precursors using complementary surfacesensitive techniques, namely quartz crystal microbalance with dissipation and atomic force microscopy. The stability of hybrid bilayers against thermal and osmotic changes is evaluated and compared to standard supported lipid bilayers formed onto hydrophilic SiO2. Force spectroscopy measurements reveal an overall weaker lateral organization of hybrid membranes as a result of the underlying self-assembled monolayer being not optimally organized. Hybrid bilayers display a decoupled behavior between the two leaflets when vertically compressed at constant speed. On microcontact printed Au surfaces, hybrid bilayers were formed over printed patches, while surprisingly, supported lipid bilayers were observed on non-patterned Au regions suggesting a non-trivial self-assembled monolayer reorganization when in aqueous environment. | Notes: | Losada-Perez, P (corresponding author), Univ Libre Bruxelles, Dept Phys, Expt Soft Matter & Thermal Phys EST Grp, Blvd Triomphe CP223, B-1050 Brussels, Belgium. patricia.maria.losada.perez@ulb.be |
Keywords: | Hybrid lipid bilayers;Atomic force microscopy;Microcontact printing;Self-assembled monolayers;Force spectroscopy;Quartz crystal microbalance with dissipation;Lipid membranes | Document URI: | http://hdl.handle.net/1942/40794 | ISSN: | 0927-7757 | e-ISSN: | 1873-4359 | DOI: | 10.1016/j.colsurfa.2023.131125 | ISI #: | 001029984600001 | Rights: | 2023 Elsevier B.V. All rights reserved. | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
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Stability of supported hybrid lipid bilayers on chemically and topographically-modified surfaces.pdf Restricted Access | Published version | 10.73 MB | Adobe PDF | View/Open Request a copy |
auteursversie.pdf Until 2025-05-05 | Peer-reviewed author version | 5.08 MB | Adobe PDF | View/Open Request a copy |
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